184 Journal of AgrictUtural Research voi. xviii. no. 4 



piled up, more shining and less wrinkled. The consistency was bu- 

 tyrous. Old cultures became dull, and the brownish tinge deepened. 



On + 10 beef-peptone agar, growth was slower and less abundant than 

 on potato agar. It was also thinner, and of finer consistency. Surface 

 irregularities were usually present as was the unevenly scalloped margin, 

 though these characteristics were not always conspicuous. The color 

 was creamy white tinged with brown. In about three days the agar 

 began to take the chestnut-brown color observed in the plate and stab 

 cultures. It was darker directly under the streak, and in a week the 

 medium usually was uniformly browned. The agar discolored more 

 slowly when cultures were grown at low temperatures or under adverse 

 conditions. 



GE1.ATIN PLATES. — At room temperature (about 21° C), the colonies 

 were usually small on -f 10 peptone gelatin plates. There was no lique- 

 faction of the medium, but it soon turned chestnut-brown in color. 



Gelatin stabs. — ^At room temperature in +10 peptone gelatin, the 

 surface growth was spreading after several days. There was no lique- 

 faction, but the medium became a deep chestnut-brown about one-third 

 of the distance down from the top. There was slight indication of 

 growth following the stab. 



Potato cylinders. — Growth on steamed potato cylinders in two days 

 was spreading, rather fiat, shining, slimy, and more or less viscid in con- 

 sistency, smooth, without odor, and yellowish white in color. The 

 growth turned the potato brownish gray. 



Milk. — Inoculated milk coagulated slowly. In a month there was a 

 fine curd developed, but no decided separation. The cultures took on a 

 cream color and had a sort of half-transparent appearance. In two 

 months a thin, somewhat clear layer developed on top, with the half- 

 transparent-looking curd below. 



Litmus milk. — Lavender-colored litmus milk cultures began to turn 

 blue at the top three days after inoculation. In six days they were 

 quite uniformly blue and remained without further change for about a 

 month, when separation occurred. In seven weeks there had developed 

 a thin, darker blue, rather clear layer on top, with fine, soft curd below 

 and a small amount of cream-colored precipitate at the bottom. 



Methylene blue in milk. — At the end of two days whitening 

 began at the bottom and proceeded gradually upward. In about two 

 weeks the cultures had become fairly white throughout with a blue layer 

 at the top only. After this the blue color returned slowly, followed by a 

 second whitening process which in about six weeks from the time of 

 inoculation was complete, except for a thin layer at the top of the cul- 

 tures. Curd began to form about this time, giving the cultures a half- 

 transparent appearance. In two months there was a thin, somewhat 

 clear, greenish blue layer on top with semitransparent, creamy, soft curd 

 below. There was no marked separation. 



