Journal of Agricultural Research voi xviii, no. 4 



however, show that there is a gradual lowering of the death point with 

 age. In a recent test (191 9) it was found that cultures from a 1915 

 strain were practically all killed at 46° to 47°, whereas trials with this 

 same strain in 191 6 had shown the normal death point to be 48° to 49°. 

 In the 1 91 9 trials all tubes of a 191 7 isolation developed fair growth 

 after being subjected to 47°, but there was no growth after 48°. Cul- 

 tures of a 1 91 8 strain showed good growth in a number of the tubes 

 after 48°, although clouding was not uniform throughout. In no test, 

 however, has there been any clouding after 49° or above. 



DESICCATION 



The organism seems not to be extremely resistant to drying as it 

 occurs in the host tissue. Isolations attempted from herbarium material 

 I and 2 years old failed to yield the organism. The vitality varies 

 on different culture media. On potato agar, where growth is very 

 abundant, the organism usually dies within two months, whereas on 

 beef -peptone agar and in bouillon it lives considerably longer. Transfers 

 made from 6-months-old cultures of these last-named media developed 

 good growth. Also cultures kept at a low temperature in a moist atmos- 

 phere, such as that afforded by an ice box, seemed to retain their vitality 

 for a much longer time than those allowed to remain at a higher tempera- 

 ture in a dry incubator. 



When dried on sterile cover glasses the organism was found to live only 

 a comparatively short time. In these tests a young, vigorously growing 

 bouillon culture was diluted with an equal amount of sterile water and a 

 2-mm. loop of this dilution transferred to sterile cover glasses which were 

 then dried in a sterile chamber. When these cover glasses were dropped 

 at intervals into tubes of sterile bouillon, it was found that the organism 

 was not alive after 65 hours of drying. 



TECHNICAL DESCRIPTION 



Bacterium glycineum n. sp.' 



Cylindrical rods, rounded at ends, solitary or in pairs; individual rod 2.3 to 3;it 

 by 1.2 to i-Sm; motile by i to several polar flagella; aerobic to weakly anaerobic; no 

 spores; capsulated when grown on blood agar. 



Superficial colonies on potato agar plates roimd, shining, convex with umbonate 

 center and surface irregularities; creamy white tinged -with brown; margin slightly 

 lobed. 



No liquefaction of gelatin; does not digest casein; nitrates not reduced; cultures 

 in various carbohydrate media produce acid with no gas. Gram negative. 



Group number, 222.2223032. 



Pathogenic on Glycine hispida Maxim., forming angular lesions which are seriously 

 destructive to leaves. Affects also pods and other aerial parts. 



Type locality: Madison, Wis., on Glycine hispida Maxim. 



Distribution: Widespread. 



> According to Migula's classification, the combination would be Pseudomonas glycineu:n, n. sp. 



