Nov. 15, 1913 



Do Mold Spores Contain Enzyms? 



205 



all the cultures inoculated with spores heated to 63° C. were a bluish-gray 

 color, and it appeared that there was considerable matter in suspension. 

 This proved to be a gum, which was precipitated by five volumes of 95 

 per cent alcohol and came down most abundantly in a strongly alkaline 

 solution. The amount of gum present increased in proportion to an 

 increase in inoculum. It was important to determine whether or not 

 the presence of this gum affected the polarization of the foregoing solu- 

 tions. Consequently the gum was precipitated from each of two 

 flasksof the loand 20per cent sugar solutions, and the sugar was filtered. 

 The polarization of this filtrate was identical with that of the unfiltered 

 solution, proving that the gum when present in such amount did not 

 influence the polarization value beyond the experimental error of the 

 method employed. The gum was found to exert no influence on Fehling's 

 solution. Further data regarding the properties and nature of this 

 gum are now being obtained and will soon be available for publication. 



Table X. — Analyses of 10 and 20 per cent sugar solutions inoculated with spores of 



Aspergillus sydowi 



,10 PER CENT SUGAR SOLUTION 



Treatment. 



10 cc. sterile water 



heated to 63° C 



10 cc. sterile water 



heated to 1 00° C . . . . 

 10 cc spores heated to 



100° C 



5 cc. spores heated to 



63° C 



10 cc. spores heated to 



63° C 



20 cc. spores heated to 



63" C 



After three hours. 



After two days. 



Polari- 

 zation. 



De- 

 crease 



polari- 

 zation. 



0-3 



•3 

 1.6 



De- 

 crease 

 in su- 

 crose. 



Per ct. 



O. 07 



07 

 40 



Polari- 

 zation 



45-6 

 46.3 

 45-6 

 44- 5 

 41. 6 



39- 7 



De- 

 crease 



polari- 

 zation. 



I. I 

 4.0 

 5-9 



De- 

 crease 

 in su- 

 crose. 



Per ct. 



0. 27 



1. CO 



1.47 



Filtrate after alcohol 

 precipitation. 



Polari- 

 zation. 



44- 5 

 44- 5 

 44- 5 

 42.8 



40.3 

 37-4 



De- 

 crease 



polari- 

 zation. 



1-7 

 4.2 



7- I 



De- 

 crease 

 in su- 

 crose. 



Per ct. 



0.43 

 I. 04 

 1.77 



20 PER CENT SUGAR SOLUTION 



10 cc. sterile water 



heated to 63° C 



10 cc. sterile water 



heated to 100° C 



10 cc. spores heated to 



100° C 



5 cc. spores heated to 



63° C 



10 cc. spores heated to 



63° C 



20 cc. spores heated to 



63° C 



72. 2 

 72. 2 

 72. 2 

 72.9 

 72. I 

 70.4 



o. I 

 1.8 



44 



72.8 

 72. 6 



72-5 

 70.9 

 69. o 

 64. o 



O. 2 



•3 



1.9 



3-8 

 8.2 



3.05 

 .07 

 ■47 

 •94 



2.05 



70-5 

 70-5 

 70-5 

 68.3 

 67.6 

 62. I 



2.9 

 8.4 



0-55 



