Nov. IS, 1919 



Do Mold Spores Contain Enzyms ? 



207 



per cent (by weight) sugar solution was prepared and diluted to con- 

 centrations of 10, 20, 30, 40, 50, and 60 per cent and sterilized in the 

 usual manner. All flasks were inoculated with 10 cc. of spores of 

 Aspergillus sydowi containing 120,000 spores per cubic centimeter. 

 The uninoculated flasks received 10 cc. of sterile water heated to 63° C. 

 After 2X days' incubation the solutions were analyzed as shown in 

 Table XII. 



Table XII. — Analyses of sugar solutions of increasing concentration inoculated with 



spores of Aspergillus sydowi 



Treatment. 



10 per cent solution, uninoculated. . 

 10 per cent solution, inoculated with 



spores at 100° C 



10 per cent solution, inoculated with 



spores at 63° C 



20 per cent solution, iminoculated . . 

 20 per cent solution, inoculated with 



spores at 63° C 



30 per cent solution, uninoculated . . 

 30 per cent solution, inoculated with 



with spores at 63° C 



40 per cent solution, uninoculated . . 

 40 per cent solution, inoculated with 



spores at 63° C 



50 per cent solution, uninoculated . . 

 50 per cent solution, inoculated with 



spores at 63° C 



60 per cent solution, uninoculated . . 

 60 per cent solution, inoculated with 



spores at 63 ° C 



70 per cent solution, uninoculated . . 

 70 per cent solution, inoculated with 



spores at 63° C 



After 2M clays' incubation. 



Polari- 

 zation. 



24. O 

 24. O 

 22.8 



48.0 

 46.4 



72. o 



71.7 



96. o 



96. o 

 120. o 



120. 



144. 



143- 

 168. 



168. 



Decrease 

 in polari- 

 zation. 



1.6 



Decrease 

 in sucrose. 



Per cent. 



0.30 



,40 



07 



Reducing 



sugars. 



Per cent. 

 15 



15 



42 

 30 



73 

 40 



43 

 51 



50 

 60 



60 

 80 



80 

 80 



80 



Increase in 



reducing 



sugars. 



Per cent. 



o. 27 

 •43 



03 



It will be seen that inversion occurred in the solutions of 10, 20, and 

 30 per cent concentration but did not take place in higher concentrations. 

 The formation of gum already described was also found at the former 

 concentrations. Again it will be noted that there was greater inversion 

 with a 20 per cent than with a 10 per cent solution. Unfortunately 

 the inoculation was too meager to produce as large a change as might 

 be desired, and it is altogether likely that an increased inoculation would 

 be more active. Thus, for the inoculum employed, the limit of concen- 

 tration appears to be between 30 and 40 per cent by weight, which, 

 recalculated to actual percentage of sucrose in the solution, is between 

 18 and 24 per cent. 



The same experiment was repeated, using the spores of Aspergillus 

 niger to the extent of 400,000 per cubic centimeter. The result is re- 

 corded in Table XIII. 



