428 Journal of Agricultural Research voi. xvm. no. s 



yeastlike budding of the fungus cells are at first round, glistening, and 

 white. Later they are red, of a gelatinous consistency, and form grape- 

 like, heaped-up pustules. As noted above, such pustules are composed of 

 mature resting spores, and when they germinate the red color of these 

 bodies is in part obscured by the mass of conidiophores produced. Colo- 

 nies formed by the toruloid method of growth are convolulted, dense, 

 homogenous, and cream-colored ; and after coalescing with other colonies 

 they form a growth such as is shown in Plate 54, A. The crateriform 

 portions of the thallus result largely from a tangential division of the 

 fungus elements within mechanically fixed borders. As noted above, the 

 resting spores in such colonies do not always reach maturity but germinate, 

 giving rise to short, subulate sterigmata, on the tips of which groups of 

 conidia are formed. The sterigmata give the thallus a woolly appearance. 



BEERWORT AGAR 



The first series of cultures of Sorosporella which were made on beer- 

 wort agar developed in a way that has since been observed only occasion- 

 ally. That is to say, the yeastlike budding process of development, 

 resulting in grapelike aggregations of mature red resting spores, took place 

 in all cultures to the exclusion of all other methods of development. 

 The resting spores did not germinate until they were almost or entirely 

 mature, so that the inoculated flasks presented a striking appearance, 

 the richly developed thallus being exclusively red in color. A portion of a 

 streak culture is reproduced in Plate 54, B ; and although the photograph 

 was taken a few days too late, when the resting spores had started to germi- 

 nate, it is possible to recognize in certain portions of it the gelatinous grape- 

 like clusters of these bodies. 



CORN-MEAIv AND OAT AGAR 



The development of Sorosporella is essentially the same on corn-meal 

 and on oat agar, on both of which it presents some phases that are 

 quite unlike those on other nutrients. At first the writer was inclined 

 to class these nutrients with potato agar as undesirable; but by keeping 

 certain cultures for a long period, a growth resulted that is especially 

 worthy of note. Subsequent inoculations have shown that this peculiar 

 phase of development occurs regularly. As on potato agar, growth is at 

 first extremely slow, practically no surface mycelium being formed around 

 the point of inoculation. After a period of six weeks, however, small 

 wartlike protuberances appeared at the bottom of the tubes, being more 

 abundant in regions where the nutrient had shrunken away from the glass, 

 and at som.e distance from the point of inoculation with no surface 

 mycelium intervening. The wartlike protuberances, in growing, grad- 

 ually coalesced, forming a prostrate, crustlike thallus, the red color of 

 which at once indicated the presence of chlamydospores. These resting 



