Jan. IS, 1920 Further Studies of Sorosporella wvella 429 



spores germinated, not by giving rise directly to sterigmata, but first to 

 hyphge, those from adjacent spores cohering. As growth proceeded, erect 

 fascicles of hyphse were thus produced which reached a height of from i 

 to 5 mm. Such a synnema is illustrated on Plate 51, G. This fasciation 

 always appears on corn-meal and oat agar if time enough is allowed. It 

 has not been observed on any other nutrient except Uschinsky's. It is 

 worthy of mention because it is identical with the growth that some- 

 times appears when an infected cutworm is opened up and the inclosed 

 resting spores allowed to germinate on moist sand (see PI. 55, A). Al- 

 though such cultures were kept for several months, no further develop- 

 ment was observed except that the conidiophores changed from white to a 

 rusty brown in color. 



uschinsky's solution with agar ^ 



For two weeks after inoculation there was no apparent growth of 

 Sorosporella on Uschinsky's solution with agar as a medium. As is often 

 the case with freshly made cultures, however, condensation water was 

 present in the tubes. When the tubes were handled this surface liquid 

 now and again washed against the bit of fungus thallus that was used to 

 inoculate the tubes, and in three weeks' time this surface liquid became 

 milky white in color and upon microscopic examination was found to be 

 full of yeastlike fungus cells. A water mount was made of these cells, a 

 photograph of which may be seen in Plate 53, B. Later the surface 

 liquid changed to a light brown tint and became viscid in consistency. 

 Without changing in other respects it gradually became darker brown 

 and more \dscid. The growth was so typically and exclusively yeastlike, 

 liquid, and unlike an)rthing before seen that a bit was transferred to 

 Molisch's agar tubes to determine whether it was an impurity. In due 

 time, however, typical grapelike clusters of red resting spores appeared, 

 indicating that these yeastlike bodies were in fact the organism in ques- 

 tion. In the meantime no change had taken place in the original tubes ; 

 but at the end of two months the yeastlike elliptical cells began to assume 

 a spherical shape, and when viewed with the naked eye a reddish tinge 

 of the thallus was observed. 



The viscid, yeastlike growth gradually disappeared and was superseded 

 by a heaped-up, pustule-like development, more intensely red in color. 

 This growth was composed of spherical, thick-walled resting spores. 

 For a considerable period there was no further change in appearance 

 of the cultures except that as additional resting spores developed 

 the heaping-up process became more conspicuous. Then germination 

 of the resting spores began, and erect fascicles of conidiophores were 

 formed in a manner comparable to that noted above. It is worthy of 



' Agar agar 15 gr.;asparagin3 to 4 gr.; ammonium lactate 10 gr.; sodium chlorid 5 gr.; magnesium sulphate 

 0.2 gr.; calaum chlorid o.i gr.; dipotassium phosphate i gr. Dissolve in 1,000 cc.water and add 40 cc. gly- 

 cerin. 



