538 



Journal of Agricultural Research voi. xvui. No. lo 



of lo to 40 .per cent solutions was made directly (using a loo-mm. 

 observation tube where necessary) while 26 gm. in 100 cc. were polarized 

 in the 50 to 70 per cent solutions. Consequently, the conversion of 

 polarization to percentage of sucrose in the 40 per cent solutions is a 

 calculation based upon an assumed Brix and specific gravity. 



Table I. — Influence of concentration on invertase activity of spores of blue aspergillus^* 



Concentration . 



Polariza- 

 tion. 



Loss in 

 polariza- 

 tion. 



Loss in 

 sucrose. 



Reducing 

 sugars. 



Gain in 

 reducing 

 sugars. 



10 per cent, control 



10 per cent, with heated spores 



10 per cent, with spores 



20 per cent, control 



20 per cent, with spores 



30 per cent, control 



30 per cent, with spores 



40 per cent, control 



40 per cent, with spores 



50 per cent, control 



50 per cent, with spores 



60 per cent, control 



60 per cent, with spores 



70 per cent, control 



70 per cent, with spores 



Per cent. 



44.2 

 44.0 



34- I 



85.1 



71.9 



124. 6 



107-3 

 166.6 



147-7 

 52.8 



47-4 

 57-7 

 54-6 

 66.1 

 61.5 



o. 2 



5-9 1-5 



13.2 3.2 



17.3 4.0 

 18. 9 4. 2 



5-4 5-4 



3-1 3-1 



4-6 



4-6 



Per cent. 

 0.03 



•03 

 1.79 



.07 

 2. 78 



. 12 

 2. 78 



.14 

 2.94 

 I. GO 

 4.41 



1. 71 

 4.17 



2. 13 



4-54 



Per cent. 



1. 76 



2. 71 



2.66 

 2.80 

 3-41 



2. 46 

 2.41 



" 144,000 spores per cubic centimeter were added to the lo to 40 per cent solutions and 130,000 spores per 

 cubic centimeter to the 50 to 70 per cent solutions. 



The results of the experiment with the spores of Penicillium expansum 

 are given in Table II. 



Table II. — -Influence of concentration on invertase activity of spores of Penicillium. 



expansum a 



Concentration. 



10 per cent, control 



10 per cent, with heated spores 



10 per cent, with spores 



20 per cent, control 



20 per cent, with spores 



30 per cent, control 



30 per cent, with spores 



40 per cent, control 



40 per cent, with spores 



50 per cent, control 



50 per cent, with spores 



60 per cent, control 



60 per cent, with spores 



70 per cent, control 



70 per cent, with spores 



Polariza- 

 tion. 



40. O 

 40. o 



24.8 



82. 5 



58-3 



122. 6 



95-3 

 170.7 

 128. 2 



53- o 

 49.9 



57-7 

 57-6 

 66.1 

 64-5 



Loss in 

 polariza- 

 tion. 



1.6 



Loss in 

 sucrose. 



Per cent. 



1.6 



Reducing 

 sugars. 



Per cent. 

 0.03 



•03 

 2.77 



•17 

 4.17 



. 21 

 5.00 



•23 



5-77 



•97 



2.87 



1. 71 

 2.63 



2. 13 

 2-73 



Gain in 

 reducing 

 sugars. 



Per cent. 



2-74 

 4. 00 



4-79 



5-24 



I. 90 



.92 



.60 



" 5,600,000 spores per cubic centimeter were applied to the 10 to 40 per cent solutions and 220,000 spores 

 per cubic centimeter to the 50 to 70 per cent solutions. 



