Feb. i6, 1920 Basal Glumerot of Wheat 547 



whole tube is clear and translucent. The color is greenish yellow. With 

 some strains this action is complete in five days, in others not until the 

 twelfth day. Thin, white rims are present, no pellicles. The sediment 

 is white, flocculent to curdy. In two months the color is light brown 

 (between "Pinkish Buff" and "Cinnamon Buff") and the medium still 

 translucent. No crystals are present. 



Litmus milk. — In 24 hours the whole tube is slightly blued, and the 

 surface shows a watery band of dark blue. This dark blue band deepens 

 until in from 5 to 12 days the whole tube is very dark blue. In undis- 

 turbed cultures there may be seen three or more distinct bands of color, 

 the darkest on the surface. In reflected light the color is almost black. 

 There is no reduction. No further change was observed in two months. 

 No crystals formed. In five different tests in litmus milk the results 

 were uniformly as stated above. In a sixth test in a medium containing 

 a smaller amount than usual of litmus, two strains (471 and 478) showed 

 a trace of reduction on the tenth day. This did not increase, and on the 

 fifteenth day the blue color was reestablished. 



Litmus agars with sugars. — On litmus-dextrose, litmus-saccharose, 

 and litmus-galactose agar the medium was reddened within 24 hours. 

 The acid action gradually increased for about 10 days then decreased, 

 until after 5 weeks there remained almost no red color. One strain 

 retained some red color in the dextrose and in the galactose media. 



Litmus-lactose agar and litmus-glycerin agar never developed any red 

 color. After five weeks the medium was a greenish blue, showing some 

 change in the litmus. 



Growth in these media was only moderate. 



Uschinsky's solution. — A moderate to heavy growth is produced in 

 this medium. Growth is best at the surface in the form of clouding, 

 pseudozoogloeae, and a delicate pellicle. The medium becomes pale, 

 apple green in color ("Veronese Green"). 



Fermi's solution. — This gives an abundant growth. A delicate 

 pellicle forms with clouding and pseudozoogloeae below. The color is 

 pale bluish green ("Pale Veronese Green"). The pellicle becomes thick- 

 er and somewhat viscid. In two weeks it is about 2 mm. thick and 

 difficult to break up. 



Corn's solution. — The organism does not grow in this medium. 



Toleration of sodium chlorid. — Peptone-beef bouillons 4-13 con- 

 taining 2, 3, 4, and 5 per cent of sodium chlorid were inoculated from 

 young agar cultures. Growth occurred in all but was rather scanty in 

 the 5 per cent. The experiment was repeated using 4, 5, and 6 per cent 

 of sodium chlorid in -f 13 beef bouillon. Again growth was scanty in 

 the 5 per cent. No growth occurred in the 6 per cent. 



Fermentation tubes. — The medium used was 2 per cent Witte's 

 peptone and i per cent, respectively, of each of the following: dextrose, 

 lactose, saccharose, maltose, mannit, glycerin, and levulose. The open 



