96 Journal of Agricultural Research voi.xii.No.a 



Rat diet A represents the mineral content of a diet which was found 

 successful for growth of pigs on artificial rations by Hart and McCollum 



(2). 



Rat diet B represents the mineral content of a diet which did not pro- 

 duce pronounced stunting in rats (4). The diet was satisfactory in other 

 factors. 



The recent work of Hogan (5) and of McCollum, Simonds, and Pitz (5) 

 indicates that the deficiencies of corn lie in the poor ash content, in poor 

 proteins and low amounts of protein, and inadequate amounts of fat 

 soluble A. 



Since our pigs on ether-extracted kernels plus corn meal made much 

 better gains than could be expected on whole com alone under these 

 conditions, our opinion is confirmed that the addition of this cottonseed 

 feed to a corn diet furnishes a greatly improved protein and a mineral 

 basis for nutrition. It is then evident that, where death ensues or poor 

 growth is manifested, this is a result of an injurious substance rather 

 than of dietary deficiencies. 



FURTHER EXPERIMENTS WITH GOSSYPOL 



Gossypol was fed to two other pigs of approximately 50 pounds' weight. 

 In one case a rather large amount was given in the ration. Pig 5 ate a 

 slop made of i pound of a mixture of corn meal and soybean meal (1:3) 

 containing 4.5 gm. of gossypol. The pig showed a poor appetite for the 

 same amount given the next morning, but ate it slowly after a pint of 

 milk was poured into it. Next, a half dose (containing 2.3 gm.) was 

 offered, one-half of which was refused. For the next five days the pig 

 was offered smaller doses, but refused it all or in part, even when tempted 

 to eat by using bran and milk in the feed. The pig showed a good appe- 

 tite for other feed that did not contain the gossypol mixture. On the 

 fifth, sixth, and seventh days the pig showed no appetite at feed time 

 but seemed sluggish and showed a desire to lie down. The pig lost in 

 weight. The experiment was then stopped. A control pig, No. 6, fed 

 on a similar ration without gossypol showed a good appetite and devel- 

 oped no such symptoms. 



After a rest of six days, the previous control pig. No. 6, was used 

 for a gossypol experiment, and No. 5 served as the control. Gossypol 

 was fed in amounts equivalent to that in 0.5 to 0.6 pound of oil-free 

 cottonseed kernels, approximately 2.2 gm. daily. An ether solution of 

 gossypol was dried on corn meal and this was mixed with more corn 

 meal and wheat bran. The other pig received the same ration without 

 gossypol. The gossypol pig ate practically all its feed for a week, and 

 then began to show a poor appetite for it and refused part. On the 

 eighth day one-half the feed was left. On the ninth and tenth days the 

 pig ate scarcely any feed. From the eleventh to the fifteenth day the 

 animal was given dry recrystallized gossypol acetate (10 per cent acetic 



