212 



Journal of Agricultural Research 



Vol. XII. No. 4 



lo pounds' pressure for 30 minutes. After cooling, two of each set of four 

 flasks were inoculated with 5 c. c. of a suspension of B. radicicola in 

 sterile distilled water. The remaining two flasks of each set (uninocu- 

 lated) served as controls. The flasks were incubated at 28° C. for 2 1 days. 

 The total weight of the flasks was maintained throughout the incuba- 

 tion period by the addition of sterile distilled water from time to time. 

 At the expiration of the period of incubation the nitrate, ammonia, and 

 total nitrogen contents were determined as given under "Methods used 

 in experiments." The contents of the duplicate inoculated flasks were 

 poured together and 50 c. c. samples drawn for analysis. The same 

 procedure was followed in the case of the uninoculated flasks. The 

 results are given in Tables XXIV, XXV, and XXVI. 



Table XXIV. — Influence of Bacillus radicicola on nitrates in solution giving the quantity 



of nitrate lost 



Table XXV. — Influe?tce of Bacillus radicicola on nitrates in solution giving the quantity 

 of nitrogen as ammonia fanned 



