152 Journal of Agricultural Research voi. xix, N0.4 



did not grow in the closed arms of tubes containing maltose or lactose. 

 In tubes containing saccharose, glycerin, and mannit there was growth 

 at first only in the open arm, with a sharp line of demarcation between 

 open and closed arms. At the end of a week clouding began to appear 

 in the closed arms of tubes containing these three substances. In 3 

 weeks there was light clouding throughout the closed arms in saccharose, 

 moderate clouding throughout the closed arms in mannit, and in glycerin 

 heavy clouding to within an inch of the top of the closed arm with light 

 clouding on up to the top. In a later test there was again light clouding 

 throughout the closed arms of tubes containing saccharose. In two 

 later tests a moderate clouding appeared in the closed arms of tubes of 

 saccharose and dextrose in from 4 to 7 days. In a later test of mannit 

 and glycerin there was no clouding in the closed arm. Tests for ammonia 

 with Nessler's reagent gave a positive reaction in solutions of maltose, 

 saccharose, mannit, glycerin, and lactose, but only traces of ammonia or 

 negative reactions in cultures containing dextrose. Titrations with 

 phenolphthalein as an indicator show a higher total titrable acidity in 

 the cultures than in the controls in saccharose and dextrose. These 

 solutions were also acid to litmus as compared with controls in two later 

 experiments. The hydrogen-ion concentrations, determined after about 

 6 weeks by the colorimetric method, were as follows: Control, Ph = 4.8; 

 dextrose, Ph = 4.8; maltose, Ph=7; saccharose, Ph=6.4; and lactose, 

 Ph=4-8. 



The organisms grew best in saccharose, levulose, and dextrose, 

 showing heavy growth in the open arm and slight to moderate growth in 

 the closed arm. This organism is evidently a facultative anaerobe 

 when certain sugars are available. 



ToivERATiON OF ACIDS. — Transfers were made to tubes of +io beef- 

 peptone broth containing o.i per cent and 0.2 per cent of citric, tartaric, 

 and malic acids. There was good growth in o.i per cent of each acid 

 but only slight growth or none at all in 0.2 per cent. 



Toleration of sodium chlorid. — Neutral beef-peptone bouillon con- 

 taining, respectively, 2, 3, 4, 5, 6, and 7 per cent of sodium chlorid was 

 inoculated from potato agar slants. There was slight clouding of 2 per 

 cent after 3 days. None of the stronger solutions clouded, but slides 

 made from a stringy white precipitate and stained with carbol fuchsin 

 showed that long chains of cells had been formed in all strengths of sodium 

 chlorid. A second test was made, using neutral broth with 0.5, i, 1.5, 2, 

 3, and 4 per cent solutions of sodium chlorid and inoculating from broth 

 cultures. There was slight clouding in i per cent at the end of 2 days, 

 slight clouding in 1.5 per cent at the end of 3 days, moderate clouding in 

 1.5 per cent at the end of 5 days. At the end of 7 days there was slight 

 clouding in 2 per cent and moderate clouding and a stringy swirl of pre- 

 cipitate in 2 per cent at the end of 19 days. Stained slides of precipitate 

 from 1.5 and 2 per cent solutions showed a network of long chains. In 

 the second test there was no growth in solutions of more than 2 per cent. 



