156 Journal of Agricultural Research voi.xix.No.4 



clouding in 2,3, 4, and 5 per cent solutions. In 3 days there was a very 

 slight clouding in 6 and 7 per cent solutions. A stringy yellow precipi- 

 tate formed in the 4, 5, 6, and 7 per cent. Slides made from 2 and 3 

 per cent solutions and stained with carbol fuchsin showed long chains 

 of cells. There were no long chains in the 4, 5, 6, and 7 per cent. In a 

 second test a delicate pink surface film, not previously observed, formed 

 in 0.5, I, 1.5, 2, 3, and 4 per cent solutions; and a pink stringy precipi- 

 tate formed in 2 and 3 per cent, becoming a brick red in 4 per cent. 



Potato cylinders. — At 25° C. there was slight growth in 24 hours 

 and a slight graying of the medium. In 4 days there was abundant 

 yellow growth, and the medium had become slightly browned. Growth 

 was filiform, flat, raised, glistening, somewhat contoured, orange-yellow 

 to red on top. There was no odor, and the consistency was butyrous. 

 There was no action on the starch. 



Milk. — Milk titrating +18 on Fuller's scale was inoculated from 9- 

 day-old potato agar slants. A slight yellow surface film was formed in 



2 days. At the end of i week yellow precipitate was evident. Curdling 

 began in 3 weeks. There was a slight separation of curd and whey at 

 the end of 2 months. The solid curd gradually dried down without any 

 evidence of peptonization. 



Litmus milk.^ — Complete reduction occurs in 24 hours, leaving the 

 medium cream -colored. Shaking tended to restore the color. After 

 about a week some of the reduced tubes were steamed, whereupon the 

 original lavender color returned. Curdling occurred in 3 weeks. There 

 was no evidence of digestion at the end of 2 months. 



Methylene blue in milk. — Reduction takes place in 24 hours. In 



3 weeks there is curdling and the blue color begins to return at the tops 

 of the tubes. No petonization. 



Uschinsky's solution. — There is moderate clouding in 24 hours at 

 25° C. and a membranous surface film. At the end of 2 days there is a 

 fairly heavy light yellow surface film. In 4 days there is heavy clouding 

 and a heavy surface film and yellow precipitate. SUdes stained Avith 

 carbol fuchsin show many short chains. 



Fermi's solution. — There is moderate clouding in 24 hours at 25° 

 C. In 2 days there is a fairly heavy light yellow surface film. In 4 days 

 the clouding is heavy and there is a heavy orange-colored surface film 

 2 mm. thick. At the end of a week this pellicle is 4 mm. thick. Clearing 

 begins in 2 weeks, and yellow strands extend from the heavy pellicle 

 to the bottom of the tube. At the end of 3 weeks the pellicle is i cm. 

 thick. In 4 weeks the medium has a greenish tinge. No chains were 

 observed on slides stained with carbol fuchsin. 



Corn's solution. — There is a slight clouding at the end of 2 days at 

 25° C. At the end of 4 days the clouding is still very light, and there 

 is just a trace of surface growth. Rhomboid crystals are formed on the 

 tube above the liquid. Growth is very slight in comparison with that 

 in Fermi's solution. 



