i88 



Journal of Agricultural Research 



Vol. XIX, No. 4 



Table IV. — Acidimetnc titrations of wheat and fi our extracts with and without alcoho 

 [50-cc. jjortians of water extract used, representing 4 gm. of sample] 



1917 series, sample No. — 



iF. 



7F. 



8F. 



1918 series, sample No. — 



1306. 



1300 

 F. 



1306 

 F. 



1307 

 F. 



Cubic centimeters of N/o.os 

 soditun hydroxid neutral- 

 ized without alcohol 



Cubic centimeters of NI0.05 

 sodium hydroxid neutral- 

 ized with alcohol 6. o 



Difference due to amino 

 compounds 



Percentage of nonprotein 

 nitrogen in total nitrogen " 



Percentage of a-amino nitro- 

 gen in nonprotein nitro- 

 gen a 



3-S 



2-5 



4. 17 



3- £2 



4.6 



S.4 

 3-8 



7- OS 



26.08 



6.8 



14.4 

 7.6 

 13.98 



36.06 



2. 2 

 0.8 



14-54 



2. o 



4.4 

 2.4 

 4.40 



29.44 



4.1 



10.0 

 5-9 

 10.56 



45-9° 



3- 1 



6.1 



3-0 



7.72 



16. 00 



3-S 



7.0 

 3-2 

 ic. 70 



4.0 



8.8 

 4.8 

 13. 20 



24.65 



I- 5 



3-0 

 1-5 

 3-05 



19.90 



2-S 

 1.4 

 3.60 



14.74 



1-7 



40 

 2-3 



5- 12 



« Figures taken from Tables I and II. 



SUMMARY 



(i) Premature freezing affects the chemical composition of wheat 

 and the flour milled therefrom. 



(2) Frozen wheat contains larger amounts of nonprotein nitrogen, 

 reducing sugars, and acid-reacting constituents than does sound wheat. 



(3) The nonprotein nitrogen of frozen wheat carries a considerably 

 higher percentage of a-amino nitrogen than that of sound wheat. 



LITERATURE CITED 

 i) BiRCKNER, Victor. 



I919. ACIDIMETRIC TITRATION OP GRAIN EXTRACTS AND AMINO-ACIDS IN THE 



PRESENCE OP ALCOHOL. In Jour. Eiog. Chem., v. 38, no. 2, p. 245-254, 

 2 fig. 



2) Blish, M. J. 



1918. A STUDY OF THE NON-PROTEIN NITROGEN OF WHEAT FLOLTl. In JoUT. 



Biol. Chem., v. 23> "o. 3, p. 551-559- 



3) Foster, Luther, and Merrill, Lewis A. 



1900. SHEEP FEEDING EXPERIMENT. Utah Agr. Exp. Sta., nth Ann. Rpt. 

 [i899]/i900, p. Ixiii-lxviii. 



4) Harper, D. N. 



1889. on the chemistry of wheat. Minn. Agr. Exp. Sta., Bui. 7, p. 65-84. 



5) Shutt, Frank T. 



1893. REPORT OF THE CHEMIST. PART I. FODDERS. In Canada Exp. Farms 

 Rpt., 1892, p. 114-121. 



6) 



1908. REPORT OF THE CHEMIST. FROSTED WHEAT. In Canada Exp. Farms 

 Rpt. [i907]/o8, p. 140-143- 



7) Stone, Winthrop E. 



1896. THE CARBOHYDRATES OF WHEAT, MAIZE, FLOUR, AND BREAD. , . . U. S. 



Dept. Agr. Off. Exp. Stas. Bui. 34, 32 p. 



