22 .lOT^ENAL OF AGRICULTURE OF P. R. 



Ihc Station, hut inorv particularly by Mr. E. G. Smyth (82) who 

 carried on work with the wliite grubs oVer a number of years. 



The insects found diseased by McforrJiizium in Porto Rieo are as 



follows: 



Aphodius sp. 



Conthon sp. 



Diiscincinft harhatus. 



Li/fiynis tumidosits. 



M eta masius hemipterus. 



Phj/Ilopliafja citri. 



Phfillopliafio fiiian iccnsis. 



Phyllophaya portorwensis. 



Phyllophaga vandinei. 



Phytalus insularis. 



Slrategus titaims. 



Tiphia inornata. (Received from Illinois.) 



The fundus has been found in addition en a number of undeter- 

 mined Searabaeids. an earwig, a roach, and some other uiuiamed 

 hosts. The localities of the many collections made have been Rio 

 Piedras f E.xpei-iuu'nt Station), Santa Rita (Guanica). and Anasco, 

 localities into which the fungus was introduced. Speeinu'iis have 

 also been taken at Fajardo {fln/Jlopliafia sp.). Hacienda Santa 

 Isabel of Agnin-e ( I'ln/llopJuifja sp.), antl Sierra de Naguabo 

 (earwig), localities into which the fungus was not introduced, a]^- 

 parently tending to prove that the fungus is indigenous to the Island. 



Fui'ther information on the stages of the various hosts attacked, 

 ]>revalence, pi'ogress of th(> disease on the individual insects, and 

 related points will he found in tiie report by Smyth (32). 



EXPERIMENTS IN ARTIPICIAI, niSSEMINATION. 



Metsehnikoff (23) conducted the first experiment with M< larr- 

 hizium, using it to fight the cockchafer of wheat. lie obtained his 

 spore material fi'om infected insects. Krassilstchik (19, 20) used 

 the fungus in a similar fashion in this work on the siigar beet curculio 

 and reported fi-oni fifty to eighty pel- cent of the insects inicctc<l. 

 These earlici- cNpcriiiients were necessai'ily on a limited scale bcc;insc 

 of the difficulty of obtaining spore iiiatci-ial in (piantity. 



During I'eccnt years extensive tests have been carried onl in 

 •lava, Hawaii, Trinidad. Porto Rico, and other regions. 



The most important of the efforts in this connection has been 

 Rorer's (27-29) work in Trinidad directed against the froghoppei- 



