CONTENTS XI 



Titration 172 



Meat Extract Broth 172 



Glycerin Broth 173 



Sugar Broths 173 



Dunham's Peptone Solution 173 



Nitrate Broth 173 



Milk 173 



Litmus Milk 174 



Gelatin Culture Medium 174 



Agar INIedium 175 



Potato Media 176 



Blood Serum Media 178 



CHAPTER XVII. 



Methods of Using Culture Media .... 181 



CHAPTER XVIII. 



Isolation of Bacteria in Pure Culture . . 191 



CHAPTER XIX. 



Study of Individual Bacteria-staining. 



Hanging Drop Shde 198 



Staining 201 



Anilin Water 203 



Preparation of the Film 204 



Gram's Method of Staining 205 



Staining of Spores in the Rod 206 



Staining of "Acid-fast" BacilH 206 



Gabbet's Method 206 



Ziehl-Neelson Method 207 



Staining of Capsules 207 



Rabiger's Method 207 



Welch's Method 207 



Staining of Flagella 207 



Staining of Metachromatic Granules 209 



Neisser's Method 209 



CHAPTER XX. 



Study of the Physiology of Bacteria . . 210 



Appearance of Growth on Different Culture Media .... 214 



