182 METHODS OF USING CULTURE MEDIA 



One method of using gelatin and also agar is as "punc- 

 ture" or "stab" cultures. The tubes (the narrower tubes 



Fig. 114.— Round wire basket. Fig. 115.— Square wire basket. 



Fig. 116. — Culture tubes with media in them. X |. 1 to 7 are the 

 smaller tubes mentioned in the text; 9 the larger tube; 8 is extra large for 

 potato tubes; 1, plain broth; 2, plain milk; 3, litmus milk; 4, gelatin for 

 "stab" or "puncture" culture; 5, agar for "stab" or "puncture" culture; 

 6, agar for "slope" or "slant" culture; 7, blood serum; 8, potato tube; 

 9, agar for plating. Note the transparency of the broth and gelatin and 

 the slight opalescence of the agar. 



