188 METHODS OF USING CULTURE MEDIA 



ized. Inoculation is made to the bottom and anaerobes 

 grow well (Fig. 123). 



D. For slope or plate, or any type of surface cultures the 

 Novy jar (Fig. 124) is the most practical device. It is good 

 practice to combine the vacuum method, the hydrogen 



Fig. 123. — Deep tubes showing anaerobic growth. 1, shows a few small 

 gas bubbles; 2, shows the medium broken up by the excessive development 

 of gas. 



replacement method and the oxygen absorption method in 

 using these jars. In operation a solution of 20 per cent 

 NaOH is poured on the bottom of the jar to a depth of 1 

 or 2 cm., the cultures are placed on glass supports above 

 the alkali and a short wide tube of strong pyrogallol is set 

 in on the bottom in such a w^ay that it may be easily upset 



