STAINING 203 



Anilin Water.— Anilin water is made by putting 3 or 4 cc 

 of anilin "oil" in a 120 cc flask, adding 100 cc of distilled 

 water, shaking vigorously for a minute or so and filtering 

 through a wet filter — in other words, a saturated solution 

 of anilin in water. 



4. Lofiler's (methylene) blue: 



Saturated alcoholic solution of methylene blue ... 3 parts 

 Aqueous solution of NaOH (or KOH), 1 to 10,000 . . 10 " 



5. Carbol-fuchsin (Ziehl's solution) : 



Saturated alcoholic solution of fuchsin 1 part 



5 per cent aqueous solution of carbolic acid .... 10 parts 

 Mix and filter. 



6. Gabbet's (methylene) blue (solution): 



Dry methylene blue 4 parts 



Concentrated H2SO4 25 " 



Distilled water 75 " 



Dissolve the dry dye in the acid and add the solution to the 

 distilled water and filter. 



Fig. 137. — Author's staining set. Square bottles are set in square holes in 

 the block. The capacity of each bottle is 30 cc. 



Staining solutions are conveniently kept in square drop- 

 ping bottles inserted in a block as shown in Fig. 137. This 



