■ Habitat Studied by . . 



. History ClassificatioD i 



MORPHOLOGY 



Underscore required terms. 



-days 



Vegetative Cells, medium used 



reaction temp age 



Form, spheres, short rods, long rods, filaments, < 



short spirals, long spirals, crtrved. 

 Arrangement, single, pairs, chains, fours, clusters, 



cubical packets. 



Limit* of length ; of diameter 



Size of majority 



Ends, rounded, truncate, concave, tapering 



Capsules, present on 



How stained 



Spokanqia, present, absent. Medium used I 



reaction temp age days \ 



Form, elliptical, short rods, spindled, clavate, drum- ^ 



sticks. I 



Limits of length ; of diameter i 



Size of majority \ 



Endospores, present, absent. i 



Method of examination, in stained or unstained J 



preparations. 



If stained, by what technic? ^ 



Location of endospores, central, excentric, subterminnt,' 



terminal. j 



Form, spherical, ellipsoid, cylindrical. 



Limits of site 



Size of majority i 



Wall, thick, thin. 



Sporangium wall, adherent, not adherent. ' 



Motility 



In broth On agar 



Flaqella, No Attachment, polar, bipolar, 



peritrichiate. How stained 



Irregular Forms 



Present on in days at °C. 



Form, spindled, cuneate, filamentous, branched, or. . . . 



I Rbactionb. 

 Gram: 1 day. . .; 2 day. 

 day. 



.Technic used. 



CULTURAL CHARACTEIUSTICS 



Underscore required 1 



Temperature 



°C. 



Age 



Growth, scanty, moderate, abundant, none. 



Form of growth, filiform, echinulate, beaded 

 spreading, arborescent, rhizoid. 



Elevation of growth, effuse, fiat, raised, convex. 



Lustre, glistening, dull. 



Surface, smooth, contoured, rugose. 



Optical characters, opaque, translucent, opal- 

 escent, iridescent. 



Chromogenesis . . .Photogenic. Fluorescent. 



Odor, absent, decided, resembling 



Consistency, butyrous, viscid, membranotts, 

 brittle. 



Medium, grayed, browned, reddened, blued, 

 preened, unchanged. 



V^ 



Nutrient 



' required terms 



Surface growth, ring, pellicle, 

 fiocculent, membranous, none. 



Clouding, slight, moderate, 

 strong, transient, persistent, 

 none, fluid, turbid. 

 Temperature I Odor, absent, decided, resembl- 



1 Sediment, compact, fiocculent, 

 granular, fiaky, viscid on agi- 



' Amount of sediment, abund- 



Growth, uniform, best at top, best at bottom. 

 Line of puncture, filiform, beaded, papillate, 



villous, arborescent. 

 Liquefaction, none, crateriform, infundibuli- 



form, napiform, saccate, stratiform; begins 



in d. complete in d. 



Degree of liquefaction in days 



Method used 



Medium, fiuorcscent, brovtmed, unchanged. 



W 



(liquid) 



Tpmperatui 



\^ 



Bsizp Characterization 

 As each of the following oharacteriatica is determined, indicate in proper marginal square by 

 means of figure, as designated below. In case any of these characteristics are doubtful or have 

 not been dctcrmiriwi, indicate with the letters U, V and X, according to the f " " 

 U, undetermined. 

 V, variable. 

 X, doubtful. 



7, Bpiral»; 8, branched i 



: 5 rods; 6, 



; § , Endospores: 0, absent; 1, central; 2, 



, absent; 1, peritrichic; 2, pola 



, negative; 1, positive 



, strict aerobe; 2, facultative anaerobe; 3, ! 



Gelatin liquefaction: 



In nitrate media: 



e nor gas; 1 , both nitrite and gas; 2, nitrite but i 



0. negative; 1, praitive 



From d-glucose: 0, no acid; 1, acid and gas; 2, acid without gas 



, nn acid; 1, acid and gas; 2, acid without gas 



From sucrose: 0, no acid; 1. acid and gas; 2 acid without gas 



! X)iameter; 1, under 0.5 p; 2, between 0.5 n and 1 



, less than 2 diameters; 2, 



i than 2 diameters 



Chains (4 or more Cflls): 0, absent; 1. present 

 Capsules: 0, absent; 1, present 



spherical; 2. ellipsoid to cylindrical 



Diameter: 1, less than < 



. ?reatt!r than dia 



mm. diameter); 3, rhizoid; 4, fila- \ 



Gelatin colonics: 1. punctiform; 2. circular (over 1 



I Acid: 0, no acid; 1, sufficient for c 



, insufficient for curdling 



, Peptonization: 0, absent; 1, present 



