“TERNSTRGIMIACE 2. Se 
For this purpose 5 grams of powdered tea is moistened 
with hot water, well mixed with one gram of hydrate of 
lime, and the whole dried on a water bath. The dry residue is 
then transferred to a small percolating apparatus and extracted 
with strong alcohol. The clear liquor is to be evaporated 
to remove alcohol, and the remaining water solution, measur- 
ing about 50 c. c., mixed with a few drops of dilute sulphuric 
acid, which separates a trace of lime and partially decolorizes 
the liquid. After filtering the slightly acid solution, it is 
transferred to a separator and well shaken with chloroform, 
which gradually abstracts the theine. This part of the opera- 
tion requires particular care, for though theine is freely soluble 
in chloroform it is necessary to shake the acidified water 
solution with several successive quantities of chloroform in 
order to remove the whole of the theine. Unless the 
quantity of theine is very large, about 200 c. e, of chloroform 
will be sufficient for 5 grams of tea, and that should be used 
in 5 or 6 separate portions, testing the last portions by distill- 
ing off the chloroform in a weighed flask until it is found that 
there is no more theine taken up. The whole of the chloro- 
_ form solution is then to be placed in a stoppered separator and 
_ shaken with a very dilute solution of caustic soda. This will 
remove a small quantity of colouring matter and render the 
theine solution quite colourless, so that on distilling off the 
choloroform from a weighed flask the theine remains in a 
condition fit for weighing. When the operation is carefully 
carried out, the theine will be perfectly white. In this way we 
have been able to obtain results of great uniformity. 
Our first ns apg were made with Indian and Cingalese 
tea, the general result showing that both kinds contained a 
much higher percentage of theine than has hitherto been 
generally supposed, and that the variation in the amount of — 
_this substance was not considerable. In this respect, however, 
