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I have experimented with tincture of Jodine on the granules 

 but got only a yellow colouring. I have made the same experi- 

 ments with the prostomial glauds and got quite the same results. 

 Evidently there is no physiological and chemical diflference be- 

 tween those glands ; I have given myself much trouble with the 

 question , whether there are anastomoses of these glandducts or uot. 

 Brandt figured the glands like grapes and the ducts themselves 

 annulated. The last is wrong, all the authors represent the ducts 

 with smooth walls. 



The glandducts are very long and can not be well isolated in 

 Na Cl solution. I tried raaceration in Ranvier's 30 % alcohol and 

 Muller' s liquor diluted with two parts of water. 



But these methods did not satisfy me and so I have chosen 

 the method of Henle for the isolation of the ductules of the 

 kidney. 



I macerated the tissue in concentrated hydrochloric acid and 

 examined in Na Cl solution (0,5 %) It is wrong to take pure water 

 for instead of 0,5 Na Cl. solution the glandducts become gela- 

 tinous in water and we are not able to isolate them. The question 

 of the anastomoses of the glandducts is very often discussed. 



Ray Lankester does not enter into details , Bertelli denies them : 

 he says to have been looking for them but did not find 

 anastomoses. Leuckart only meutious anastomoses near the roots 

 of the denticules. Vogt and Jung describe and figure two 

 glandular cells with a common excretory duet. Often I thought 

 to have found anastomoses, but more careful examination with 

 high magnifying power taught me , that the ducts were kitted 

 together for a remarkable length and were not to be separated 

 even by strong currents in the liquid. 



Often however I was lucky enough to find real anastomoses, 

 the ducts of two glands were fused to one excretory duet and 

 it was impossible to separate them by strong movement of the 

 liquid, moreover an examination with Apochr. 3. 1,40 hora. Imm. 

 Oc. 1 2 (Zeiss) taught that there was only one single duet , after 

 the fusion. 



