T- 



OaCEWEJE. 395 



aqueous solution of the alcoholic extract, the liquid formed an 

 emulsiou which showed little or no tendency to separate. The 

 amylic alcohol tincture was evaporated on a water bath, and, 

 when dry, was repeatedly agitated with ether, until colouring 

 matter ceased to be dissolved. The extract insoluble in ether 

 was then redissolved in amj^ic alcohol and agitated repeatedly 

 with baryta water^ until the baryta water ceased to be colored 

 yellow. During agitation a soft varnish-like mass sei)arated 

 and adhered to the sides of the bottle. By this treatment the 

 original amylic alcohol extract was separated into three fractions : 

 (1) The amylic alcohol solution, (2) the varnish-like residue 



solution - 



bottle 



(1) The amylic alcohol solution on evaporation left a solid 

 residue, which, after being pounded, and agitated with ether, to 

 remove traces of adherent amylic alcohol, possessed the proper- 

 ties of a saponin-like principle ; it frothed considerably with 

 water; treated with concentrated sulphuric acid, a dirty reddish 

 coloration was slowly developed; in water and aqueous 

 ammonia it was only slightly soluble, but dissolved easily in 

 ordinary acetic acid. As extracted the principle was not pure, 

 it contained colouring matter and barium. 



F 



(2) The varnish-like residue was dissolved in acetic acid and 

 agitated with amylic alcohol, the extract being treated with ether 

 to remove traces of amjdic alcohol. This extract also behaved 

 like a saponin-like principle : after purification it formed a 

 yellowish powder, it frothed considerably with water ; treated 

 with concentrated sulphuric acid, it developed in a shorter period 

 than the first extract a beautiful bright carmine coloration: in 

 water it was easily soluble, a concentrated solution having 

 much the physical appearance of an aqueous q^^ albumen, and 

 it dissolved readily in aqueous ammonia. 



(3) The baryta water solution contained much colouring matter 

 and a small amount of a principle which frothed with water, 

 which was probably a mixture of the two princii^los already 

 mentioned. 



