APPENDIX. 107 



The dialysate was next evaporated down in portions to about two 

 litres upon a water-bath, and tlie resulting deposit, consisting of 

 colouring and other organic matter, as well as some calcium meco- 

 nate, removed by filtration. The filtrate reacted acid to litmus, and 

 m it were detected morphine, narcotine, narceine, codeine, sulphuric, 

 and meconic acids. It was next acidified with hydrochloric acid, 

 and after heatino: on the water-bath was treated with a boiling 



solution of barium chloride in excess. After standing over night the 

 resulting barium sulphate was filtered oS and washed out with hot 

 water containing hydrochloric acid until it was white. It was then 

 dried, ignited, and weighed, and yielded^ with the portion that was 

 similarly treated separately, the following figures : 



Portion I— B^iSO* ... 2-9236 grama 



Portioa II— ;, ,., .., ... 0-3020 „ 



Total... := 3-3156 



^ . , ^ ^ ( l'''^9i5 grams H^SO*, ) 



Equivalent to ^ ^^ ^,^^^^ ^^ SQ5 | . 



The filtrate from this precipitate was neutralized and precipitated 

 in the cold with ammonia which was added in slight excess. After 

 standing for several days, the ju'ecipitated alkaloids were filtered off 

 and the filtrate again made aramoniacal and left to stand, when more 

 alkaloid was precipitated. This was continued until the resulting 

 filtrate no longer gave a reaction for alkaloids. The various pre- 

 cipitates were then filtered off and dried at SO"" C. to constant 

 weight, and regarded as the total alkaloids of the opium taken. 



They were then treated for several days with an excess of lime- 

 water until this took up no more alkaloid. The remaining alkaloids 

 Were then filtered off, washed with slightly ammoniacal cold water, 

 and dried at 80° C. They were then weighed and regarded as narco- 

 tine. The results obtained are given belo^w 



Porcelain dish + alkaloids (total) ==24-302;5 gr. 



I» M 



alone - - - • • ■ " • •■ • 



« •« 



14-0465 gr. 



Hence, total alkaloids fomd = 10*2558 gr. 



Karcotine (weighed on tared filter) - 4-3631 



giving as the final result 



Morphine, 5-8927 grams, equivalent to n'79peroeat. 

 Narcotine, 4-3631 ,» n ^^ 8-i3 „ „ 



The other alkaloids present in opium, such as codeine, narceine, 

 papaverine, &c., were not considered separately, as they, in aU 

 probability, play the same rSle with respect to the acids present as 

 does morphine. 



