ArPENDIX. 



175 



Reagent. 



Brucine. 



Alkaloid suspected to 

 be Erucine. 



Bichromate of potas- 

 sium in acetic acid 

 sola Hod. 



Platiuic chloride 



Aiu'ic chloride 



« •« 



Copious yellow ppt., with 

 difficulty soluble iu large! 

 excess of acetic acid. I 



Thick yellowish iloc^ ppt,, 

 with difficulty soluble in 

 acetic acid, but with, ex- 

 ception of a few flocks 



completely soluble in 

 NaHO. ^ 



Dirty white floe* ppt,, soon 



chan<ring to flesh colour, 



soluble iu excess of aoetic 



Copious yellow ppt., with 

 difficulty soluble in large 

 excess of acetic acid. 



Thick yellowish floe, ppt., 

 readily soluble in acetic 

 acid, but almost insolu- 

 ble in NaHO. 



Potassium ferrocyan- 

 . ide. 



Alcoholic solution ot\ 

 iodine. 



acid, bat insol. in NaHO< 



Light yeUow ppt., soluble 

 in dilute 11' ^OK The 

 presence of acetic acid 

 in slight excess prevents 

 precipitation. 



Alcoholic solution of alka- 

 loid, rosette crystals. 



Beautiful purplish- red ppt,, 

 soon changing to dirty 

 brown, mth a green tinge, 

 sol. in excess of acetic 

 acid, but insoL in NaHO. 



Li^ht yellow ppt., sol. in 

 dil IPSO*. The presence 

 of acetic acid in slight 

 excess does not prevent 



precipitation. 

 Alcoholic solution of alka- 

 loid, no crystalline f onna 

 on microscopic examina- 

 tion. 



T\vo experiments were made to determine whether the alkaloid 

 possessed any physiological properties similar to brucine. In the 

 first experiment "15 gramme was dissolved in three drops of acetic 

 acid dihited with about two di'achms of water, and injected into a 

 cat's stomach at 11-21 a^m. 



11-37 A.M. 



vomited 



was a good deal of frothy mucus and constantly dribbling saliva, 

 movement of the jaws, and application of the paws to the mouth as 



if to remove some irritant matter; the animal restless and much 



distressed. 



12-30 P.M. — Filthy mucus and saliva still flowed from the 

 mouth, but in smaller amount ; vomiting ceased, but now and then 

 retches ; animal not so restless. 



saliva ceased; animal quiet, no further 



1 P.M. 



Discharge of 



symptoms developed. 



In the next experiment '022 gramme oi the alkaloid was dissolved 



drj 



the residue dis- 



solved in a few drops of distilled watex-, and the solution hypoder- 

 inically injected into the left hind leg of a small frog at 11-40 a.m. 



