184 AiTENDIX. 



4 



In addition to the seed tinctures, we also i^rcparcd, for the purpo"^. 

 of comparison, three scries of tinctures from the leaves, menstrua of 

 the same alcoholic strength, and the same proportion of drug to 

 menstruum being used, as in the case of the seed tinctures. 



Before proceeding to the quantitative estimation of the alkaloid, a 

 few preliminary cxpcrimcuts were tried, in order to ascertain whether 

 the process employed for the estimation of the alkaloid in tinctures 

 of henbane and belladonna was equally well adapted for the 

 estimation of the stramonixmi tinctures, 



L 



For this purpose 300 c.c. of a standard tincture was prepared 

 with a GO per cent, menstruum, and the alkaloid estimated by the 

 following processes, the usual precautions being taken to prevent 

 loss of alkaloid in washing with chloroform, &e. : 



Exjjcnmcnt /, — Fifty c.c. of the tincture Avas evaporated to low 

 bulk with addition of water, until all spirit had been removed. The 

 residual liquor was allowed to cool and was then acidified with 

 dilute sulphuric acid, and freed from fat and colouring matter by 

 means of chloroform. It was then made alkaline, and the alkaloids 

 removed by shaking Avith three successive portions of chloroform. 

 From the mixed chloroformic solutions the alkaloids were extracted 

 by three agitations with acidulated water, and were afterwards 

 regenerated from the mixed acid solutions, after addition of excess 

 of ammonia, by shaking out Avith chloroform. The latter solution 

 was then shaken with ammoniatcd water, and after separation was 

 drawn off and evaporated, and the residue dried at 100^ and 

 weighed. 



Experiment II, — The tincture was evaporated to low bulk, the 

 residual liquor allowed to cool, and an excess of dilute sulphuric 

 acid added. It was then freed from fat and colouring matter by 

 means of chloroform, a slight excess of ammonia added, the alkaloids 

 shaken out with three doses of chloroform, the latter solutions 

 mixed, and after treatment with ammoniated water evaporated, and 

 the residue dried and weighed. 



Experiment III — The tincture was evaporated till all spirit was 

 removed, and the residual liquor acidified with dilute sulphuric acid 

 and shaken with chloroform and ether iu turn, till the latter came 

 away colourless. The alkaloidal solution was then made alkaline 

 "With ammonia, the alkaloids shaken out with throe <^ucccssivo 15 e.c. 



