APPENDIX, |g5 



ether (sp. gi\ •717), the mixed ethereal solutions evaporatea, and 

 the residue dried and weighed. 



Experiment IF. — The tincture was evaporated to remove the 

 spirit, the residual liquor acidified, and colouring matter removed by 

 means of chloroform. It was then made alkaline and the alkaloids 



» 



extracted with chloroform- From the chloroformic solution the 

 alkaloids were removed by agitation with 'acidulated water, the 

 latter solution treated with a slight excess of ammonia ; the alkaline 

 liquid shaken with three successive portions of ether (sp. gr. "717), 

 and the mixed ethereal solutions evaporated, and the residue dried 

 at 100°, and weighed. 



Experhnent V. — This was conducted exactly as No. IL, the oil 

 being removed by means of petroleum ether before the preliminary 

 treatment with chloroform. 



The results were as follows : 



Experiment I. 50 c.c. tincture = -014 m-am alkaloids. 



?> 



3> 



Experiment IL 50 c,c. „ = -015 



Experiment III. SOc.c. „ — '012 



Experiment IV. 50 c.c. „ — '010 ,, 



Expei'iment V, 50e.c. ,, == '014 „, 



F 



These results indicated that the method of extraction by means of 

 chloroform was thoroughly reliable^ notwithstanding the presence of 

 fixed oil in the tincture, and also showed that the preliminary 

 treatment with petroleum ether, in order to remove the oily matter 

 was unnecessary. Confirmatory experiments with a 70 per cent, 

 tincture, by processes II. and V., gave the following residts : 



No. II. 60 c.c. tincture = •QlS gram alkaloids. 



No. V. 50 c.c. „ ^ 014 



; y 



The exact details of the process adopted are as follows : 



Fifty c.c. of the tincture to be estimated is inti^oduced into a por- 



celain dish, and evaporated over a water -bath to low bulk ; water 

 being added, if necessary, until all the spirit is removed. The resi- 

 dual liquor is allowed to cool, and is tlien acidified by the addition 

 of 1 c.c. semi-normal sulphuric acid, and the liquid filtered through 

 cotton wool into a separating funnel. The dish and filter ai-e rinpcd 

 first with a little acidulated water and then with 15 c.c. chloruform, 

 the rinsin;rs added to the contents of the funnel and the whole 



