R. K. Nabours 157 



leucoiK^tus-nigronotatus (BI) individuals thab have since been produced. 

 Their F, progeny was as follows (Table III (e), F^) : 



Leuconotus- Leuconotus- Leucothorax- 

 leucothorax luteolineatus nigronotatus 

 {BO (BE) [CI) 



Actual Numbers 12 11 7 



Expectation 12-25 12-25 12-25 12-25 F, 



This result is perfectly regular in every respect, except tor the 

 appearance of the leuconotus-luteolineatus-nigronotatus (BEI) in- 

 dividual. This specimen was observed during its second instar when 

 its characters were perfectly distinct, and it was kept under clo.se 

 observation until it became adult. The pattern was distinct at all 

 times, but was strikingly clear just after moults. Unfortunately it 

 escaped while the culture was being transferred from the University 

 of Chicago to the Kansas State Agricultural College, at Manhattan, 

 in September, 1910. It seems that the production of this aberrant 

 individual may be accounted for by assuming that the leuconotus- 

 nigronotatus {BI) female parent gave at least one gamete containing 

 the factors for the patterns of both of her parents and that this double 

 character gamete was fertilized by one of the luteolineatus (E) gametes 

 which came from the leucothorax-luteolineatus (GE) male'. 



(4) Further hyhidization and inbreeding of the resulting hetero- 

 zygotes. 



E.Tperimeiit IV. The following are additional data concerning the 

 simple crossing of forms where the composition of one of the parents 

 was known and that of the other parent unknown. 



(a) A male nigronotatus (Plate VI, fig. 9), from nature, whose 

 pedigree was not known, and a female leuconotus, fiom the F.^ genera- 

 tion of a pure leuconotus culture, were crossed, and they gave in Fi ten 

 adults, all leuconotus-nigronotatus(i^/) heterozygotes (Table IV (a), F^). 

 A pair of these were inbred and gave in F., three types as follows 

 (Table IV (a), F,) -. 



Leuconotus 



Leuconotus nigronotatus Nigronotatus 

 IB) {BI) (/) 



Actual Numbers ... 3 13 5 



Expectation ... 5-25 10-5 5-25 



' In subsequent experiments — the production of an indindual with the patterns of 

 three of its ancestors combined — has been repeated four times with other combinations of 

 patterns. I now have leuconotus-rufrolineatus-melauothorax (BFG), leucothorax-rufro- 

 lineatus-melanothorax (CFG), leueonotus-leucothorax-melanothorax [BCG), and rufro- 

 lineatus-melanothorax-leuconotatus (FGJ) (the leuconotatus (J), a new form to be 

 described later). These have now been bred for some time, some of them to the F^ 

 generation. TLeir inheritance behaviour will be reported soon in another account. 

 Journ. of Gen. iii 11 



