JOURNAL OF THE 



[January, 



ON CULTIVATING LIVING ORGANISMS UNDER 

 THE MICROSCOPE. 



BY E. A SCHULTZE. 



[Abstract and translation from an Article by Dr. John af Klercker in Zeitschrift 

 fiir Wissenschaftliche MikrosJcojne, vi. 2, p. 145 (1889).] 



{/Ceat/ October \%tlu l88q.) 



Any one, at all familiar with the microscopical study of 

 living organisms, will have met with the difficulty of making a 

 satisfactory examination, on account of the globular shape of 

 the liquid containing the culture. It is well known that, in 

 order to bring the objects to a full state of development, the 

 water, especially in the case of algre, must often be changed. 



In doing this, it is in most cases difficult to prevent the algre 

 from changing their position under the cover-glass. Con- 

 sequently a filament under observation may be lost to view. 

 Sometimes, when a too sudden evaporation is feared, the volume 

 of the drop must be increased to such extent that the high- 

 power objectives, and especially the homogeneous immersion 

 lenses, can only be used on those filaments which lie nearest to 

 the cover-glass. 



To obviate this difficulty I have been using an apparatus, 

 the construction of which admits of ^ constant flow of water, 

 and I find that it answers to my entire satisfaction in every 



