INVERTEBEATA, CRYPTOGAMIA, MICROSCOPY, ETC. 287 



tlie microscopic organisms whicli live in the same water as Infusoria. 

 The Rotifers, Entomostraca, Anguillulce, and Entozoa are deeply 

 coloured by the iodine. The characteristic colour is always more or 

 less localized in certain organs. The Bacteria and Vihriones are never 

 coloured. Amongst the smallest Monads and Flagellates some take 

 a mahogany-brown colour, others turn to a violet-black, whilst some 

 remain colourless. The protoplasm of Alga3 and of vegetable cells 

 in general becomes slightly yellow. The hyaline sphere of the 

 Volvociuese does not appear to undergo any modification. 



The author says that the question may be perhaps asked whether the 

 presence of animal starch may not constitute that criterion, so long 

 sought for in vain, defining the limit between the animal and vegetable 

 kingdoms, but for the elucidation of this question fui'ther experiments 

 are necessary. 



New Species of Ophrydium.* — Dr. H. C. Evarts describes a 

 second species of Ophrydium (0. Adce), very closely allied to 0. versa- 

 tile. The generic and specific characters are the following : — Lorica 

 gelatinous ; animals radiately clustered in numbers varying from one 

 hundred to four or five hundred or even a thousand ; each one attached 

 by a long, non-contractile stalk, which penetrates to the interior of 

 the jelly-mass. The expanded body extends beyond this mass; when 

 fully contracted it is drawn down into it. The extended body is 

 elongated, ta2:»ers gradually from its point of greatest diameter, which 

 is in the inferior third of the body, to a small, rounded, inferior 

 extremity. Above the dilated portion, the diameter, after diminish- 

 ing for a short distance, becomes uniform almost up to the mouth, 

 where it expands slightly. Peristome annular ; disk well elevated, 

 and higher on one side than on the other. Endoplast or nucleus 

 very long. 



The -iiliated oesophagus extends half-way to the contractile vesicle. 

 The external surface of the body exhibits very fine, transverse 

 striation. During contraction, this striation is not more marked, 

 except in that part below the dilated portion, and is much less 

 consjiicuous tlian in 0. versatile. Moreover, during partial or complete 

 contraction, the appearance presented is not the same as in the latter; 

 the " flask-shape " is never assumed. There arc no longitudinal folds 

 like those of 0. versatile, except when the animal is subjected to 

 pressure, and even then they arc not always produced. 



The animals were not seen encysted, nor in the acinetiform phase 

 that Stein has observed in 0. versatile. They are not of a vivid greea 

 colour ; the chlorophyll granules are few, mostly collected in the 

 dilated portion. When fully contracted the body is pyriforra, or 

 nearly oval in shape. The animals are associated in smooth, globular, 

 transparent, homogeneous masses, which are always attached, varying 

 from one half-lino to two lines in diameter. 



Length of extended individuals about yxhs inch. Habitat, so far 

 as known, only fresh water. Although the locality was frequently 

 visited during the spring and sunnner, and the fauna of the stream 



• 'Am, M. Micr. Journ..' i. (1S80) p. 1 (5 figs.)- 



