October, '14] TOWNSEND: VERRUGA AND PHLEBOTOMUS 359 



and blood normal. Injected subcutaneously September 4 with 75 

 Phlebotomus in physiological solution. Marked leucocytosis shown 

 in smears of September 5 to 7 inclusive, especially latter date, white 

 cells becoming normal on September 8. Smears of September 6 and 7 

 show Bartonia bodies. September 5 to 8 the temperatures varied from 

 39.2° to 40.2° on latter date, and averaged .7° above normal for two 

 weeks thereafter. September 6 the animal weighed only 3,900, was 

 decidedly ill, thin, with no appetite^. Weight gradually increased 

 after that date, appetite improving on following days. No visible 

 external sign of eruption has appeared. This seems a case of cutting 

 short the disease by phagoc3'^tosis, at least to an extent, in an animal 

 not particularly susceptible but rather resistant. 



Experiment VI 



Lepus cuniculus, male, about 1 year, brought to laborator}- March 

 29 from Jauja, 11,000 feet. Temperature average under 39°. Normal 

 weight 1,700. Healthy, blood normal. Kept in cage August 7 to 11 

 with nine living Phlebotomus, most of which died on second and third 

 days. Injected subcutaneously August 8 with 21 Phlebotomus in 

 physiological solution. August 9 to 14 temperatures varied from 38.4° 

 to 39.5°, weights falling to 1,588 on latter date. Smear of August 11 

 shows Bartonia bodies and marked leucocytosis which continued till 

 August 13, leucocytes becoming normal the next day. August 16 

 smear also shows Bartonia bodies, but white cells were normal. Tem- 

 perature was below normal on that date, after which weights and 

 temperatures approximated normal and no visible external eruption 

 appeared. On October 16 the animal received a scrotal injection of a 

 small quantity of virus from nodular verruga eruption of man. The 

 result of latter inoculation was a small scabbed verruga at point of 

 injection, persisting from November 4 to 26. This appears to be a case 

 somewhat similar to No. II, the verruga organisms being largely 

 phagocyted out of the system following the infection with Phlebo- 

 tomus. That such course does not confer immunity is indicated by 

 the localized verruga following the inoculation two months later with 

 virus from man. 



Experiment XV 



Canis caraihicus, female, about 18 months, from Chosica. Admitted 

 to laboratory April 24. Average temperature under 38.5°. Normal 

 weight about 3,500. Perfectly sound, blood normal. Injected sub- 

 cutaneously July 11 with 20 Phlebotomus in physiological solution. 

 The course of this case up to July 19 is published in the Journal of the 

 American Medical Association (Nov. 8, 1913). Nodular eruption on 



