360 JOURNAL OF F.rOXOMIC ENTOMOLOGY [Vol. 7 



feet appeared July 17, the animal having been decidedly ill the day 

 before and showing Bartonia bodies in the blood. Temperatures did 

 not rise appreciably, being less than a degree above normal average. 

 Weights slightly lowered. Papules on feet receded and renewed, some- 

 times bleeding, up to August 29, when weight fell to 2,957 and erup- 

 tion distinctly decreased in intensity, remaining so for some days. 

 September 4 the extreme tips of both ear-lobes showed eruption, 

 scabbed, bleeding more or less, of the excrescentic-nodular type, con- 

 tinuing on both ears till September 29, and on right ear till October 

 16 when it had nearly disappeared. Sections of ear tips and papules 

 show portions with apparent histology of verruga eruption. On Octo- 

 ber 16 this dog received five separate subcutaneous injections, in dis- 

 tinct regions, of virus from nodular verrugas of man. Absolutely no 

 result followed these five inoculations, no lesions forming at points of 

 injection. This indicates immunity conferred by the Phlebotomus in- 

 jection of July 11 followed by a generalized eruption. It is quite 

 possible that internal eruption may also have ensued, as indicated by 

 continued loss of weight and more or less pain in joints and body. 



Experiment XVI 



Canis caraibicus, male, about 2 years, from Chosica. Admitted to 

 laboratory April 24. Average temperature under 38.5°. Normal 

 weight about 6,300. Sound, blood normal. This animal received 

 five separate subcutaneous injections on September 19, 23, 25, 27, 

 and October 12, with 80, 75, 107, 109 and 50 Phlebotomus respectively 

 in phj'siological solution, the injections alternating between the two 

 shoulders. Smears showed Bartonia bodies September 20 (22| hours 

 after first injection), September 25, and again October 14. They 

 showed leucocytosis beginning September 27 and 28, increased Sep- 

 tember 29 and 30, white cells normal again October 1 and continuing 

 so until October 13, when another increase was noted, which had dis- 

 appeared next day. Polychromatophilia of the erythrocytes was 

 evident September 21 to 25. Temperatures ran 37.7° to 40.6° from 

 September 20 to October 7, but only rarely fell below 39° and were 

 nearly always close to 40°. October 7 to 12 temperatures decreased 

 some, going below normal on latter date and remaining so continu- 

 ously till November 2, except only October 20 and 27. Weights 

 gradually dropped to 5,808 September 28, with temperature of 40°, 

 and remained under 6,000 to September 30 inclusive. Abscesses of 

 considerable size formed at points of the five injections, but all were 

 absorbed except the fifth which broke October 18. November 2 a 

 nodular verruga suddenly formed on dorsa median line between shoul- 

 ders, and increased in size during next two days, being of large size 



