October, '14] TOWNSEND: VERRUGA AXD PHLEBOTOMUS 361 



with angular scabs on November 4 when it was excised. Another 

 verruga at once began to form in its place, reaching good size November 

 10 and continuing with slight further growth to December 15 and later. 

 A small typical nodular verruga formed November 20 to 22 on base 

 of an ordinar}' wart which had sloughed on former date. This was 

 on right chest and the verruga was excised November 22. Sections 

 of these two excised verrugas show the identical histological structure 

 of nodular verrugas from man. To sum up, this dog has shown de- 

 crease in weight, marked rise and fall of temperature, Bartonia bodies 

 in blood, with spare leucoc^-tosis and erythrocj-tic polychromatophilia, 

 followed by what must be considered a generalized though meager 

 eruption, as result of injection of over 400 Phlebotomus from Verrugas 

 Canyon. Internal eruption may also have ensued in this case. This 

 is the star injection experiment, and the one which has afforded the 

 most convincing results. 



Experiment XIX 



Cavia cobaya, male, born in laboratory May 13 of parents from .Jauja. 

 Average temperature 38.5°. Average weight about 400. Perfectly 

 healthy and blood normal. Placed in cage July 29 with two living 

 Phlebotomus, to which were added six from Matucana on August 2, 

 being removed finally from cage August 7 on death of the last Phle- 

 botomus. Smears of August 7 and 16 show Bartonia bodies. A marked 

 lymphocytosis was evident August 8 and 9, and again August 19 and 

 20, unusual numbers of small hmiphocj-tes showing in the smears, 

 being normal on other dates. Temperatures showed an appreciable 

 continued rise for six weeks after August 2, being practically all 

 (a. m. and p. m.) between 39° and 40°. No visible external eruption 

 followed. This is another case of an animal with small susceptil)ility 

 phagoc\i:ing the organisms and showing no external eruption. The 

 infection was undoubtedly slight. 



Experiment XXII. 



Cavia cohaya, female, born in laboratory May 18 of parents from 

 Jauja. Normal temperature 38.5°. Placed in cage July 24 with 12 

 living Phlebotomus, and left there till July 29 when it died, 2 of the 

 Phlebotomus being still alive. Smear taken July 28, at 10.15 a. m., 

 shows Bartonia bodies, as does also smear of autopsy thoracic-cavity 

 blood. Autopsy liver, spleen, cord and lung smears show interesting 

 conditions which require further study. Temperature at 9.30 a. m., 

 July 28, was 39.8°. That this pig died from the infection induced by 

 the bites of the Phlebotomus is not at all certain. Yet the temperature 

 was high the day before, and autops}^ of the digestive tract disclosed 

 nothing abnormal. 



