362 JOURNAL OF ECONOMIC ENTOMOLOGY [Vol. 7 



Experiment XXIII 



Cants caraibicus, male, about 5 months, from Chosica. Admitted 

 to laboratory July 25. Average temperature under 38.5°. Normal 

 weight about 2,900. Animal perfectly sound. Smear showed blood 

 normal before experiment. Injected subcutaneously in right shoulder 

 July 25 Avith 25 Phlebotomus in physiological solution. At 5 p. m., 

 six hours after injection, temperature was 40°. Smears of July 27 

 (48 hours after injection) and August 20 show Bartonia bodies. Tem- 

 peratures averaged a little above normal from July 25 to August 2, 

 going from 39.3° to 40° during the first three days. Throughout Aug- 

 ust they were usually below normal, rarely above, after which they 

 gradually rose to around normal. Weight July 26 was 2,041, and 

 varied through August from 2,496 to 2,839, gradually rising. Leu- 

 cytosis showed in smear of July 28. Small sores appeared suddenly 

 on ear-lobes August 23, these bearing considerable resemblance to 

 miliar eruptive sores, but only slightly raised. They continued on the 

 ear-lobes, inside and out, increasing, drying, decreasing, and coming 

 anew, until September 16 when most of the scabs had dropped. By 

 October 6 there was no trace of the scars. Sections of these ear papules 

 show a strong approximation to verruga histological structure. On 

 October 12 injected subcutaneously in right shoulder mth 50 Phle- 

 botomus in physiological solution. Temperatures averaged but 

 slightly above normal for three weeks following this last injection, but 

 at times passed 39° and 40°. Weight increased gradually, and smears 

 apparently showed neither Bartonia bodies nor anything abnormal. 

 December 2, however, a small, hard, raised, uncolored nodule appeared 

 suddenly on outer base of left foreleg, and by December 5 had become 

 a typical hard nodular verruga to all appearances. It was excised and 

 sectioned for study. The sections show a typical verruga histology. 

 If the ear sores above described were verruga, as is probable, they did 

 not confer immunity in this dog. 



Experiment XXV 



Canis criollus (tan-haired creole dog), male, about 6 months, from 

 Chosica. Admitted to laboratory August 16. Average temperature 

 under 38.5°. Normal weight about 3,800. Healthy, and smear showed 

 blood normal before experiment. Placed in cage September 18 with 35 

 living Phlebotomus, to which were added 25 on September 23; 30 more 

 on September 25; and 30 more on September 27, all from Verrugas 

 Canyon. Removed from cage September 29, the Phlebotomus being 

 practically all dead, notwithstanding the daily envelopment of the 

 cage with wet cloths in the effort to prolong their lives, and the con- 

 finement of the dog between two wire desk baskets to prevent free- 



