364 JOURNAL OF ECONOMIC ENTOMOLOGY [Vol. 7 



ing off and on till December 15, and later, with eruption lesions appear- 

 ing on throat December 1. Smear of October 17, taken 24 hours after 

 the virus injection, showed Bartonia bodies and leucocytosis. It may 

 be stated here that the abscess in this and other cases was not due to 

 defective technique, aseptic methods having been employed in all 

 cases. The present very large abscess was doubtless due to septic 

 material from the decomposition of tissues within the unbroken ver- 

 rugas from which the virus was taken. This experiment indicates 

 a certain degree of control of the infection from the Phlebotomus in- 

 jection, no immunity being conferred; but inability to control the 

 infection from the virus injection, which resulted in a generaUzed 

 eruption, as distinguished from a purely localized one at point of 

 injection. 



Experiment XXVII 



Canis caraibicus, male, about 18 months, from Lima. Admitted to 

 laboratory August 28. Healthy, blood normal. Injected subcu- 

 taneously with 39 Phlebotomus in physiological solution, August 29, 

 at 11.30 a. m. Temperatures rose on succeeding dates, reaching 39.5° 

 on September 2. Weights decreased steadily from 3,575 on August 

 30 to 3,357 on September 3, except 3,646 and 3,605 on September 1 

 and 2, Smear of August 31, 46 hours after injection, shows Bartonia 

 bodies rather numerously, also some leucocytosis. Dog very sick 

 and weak August 30 to September 2, without appetite August 30 and 

 31. Chloroformed September 3 and various tissues preserved for 

 sectioning. 



Experiment XXVIII 



Canis caraibicus, male, about ten months, from Chosica. Admitted 

 to laboratory September 10. Average temperature well under 39°. 

 Normal weight about 4,500. Healthy, blood normal before experi- 

 ment. Testicular injection given at 10.30 a. m., October 5 with 100 

 Phlebotomus in physiological solution. Smear of October 6, taken 

 at 8 a. m., shows Bartonia bodies, as do those of October 8 and 9. 

 Weights decreased but temperatures remained practically normal. 

 Abscess formed in testicle and broke October 9. Leucocytosis ap- 

 peared in smears of October 9 to 11. Chloroformed dog October 12 

 and preserved tissues for sectioning. 



This concludes the experiments to date. The writer wishes to 

 acknowledge the valuable service rendered by his assistant on this 

 work, Mr. George E. Nicholson. Unfortunately Mr. Nicholson con- 

 tracted the disease as the result of being bitten by the Phlebotomus on 

 the night of September 17 at Verrugas Canyon, an account of which 

 was sent to Entomological Neios (vol. XXV, p. 40). His fever continued 



