116 



COMBINATION OF ENZYME AND SUBSTRATE 



Readings were taken at intervals so as to give points corresponding 

 to changes of 2 to 4 units of the bridge reading (which could be easily- 

 read to 0.25 units). These points were then plotted on a large scale 

 by means of a flexible "spHne" and weights, so that the curves were 



TABLE I. 



Change in Conductivity of Solution of Egg Albumin with Varying Amounts of Pepsin. 



25 cc. of egg albumin solution, pH 2.6. R 320. 

 Temperature 37° ± 0.02°C. ^ at beginning 500. 



Relative amount of pepsin added in volumes of 1 cc. 



30 to 50 cm. long. The time necessary to cause a given change was 

 then interpolated from these curves. In this way errors in individual 

 readings were smoothed out and, as the curves are regular, accurate 

 values for the time necessary to cause a given change could be ob- 



