330 REGENERATION AND NEOTENY 



recorded individually. In Series LVI the fore limbs were removed 

 at 26 days, the hind limbs at 61 days, and 11 cm. of the tail at 47 days 

 after hatching. To assure continuous regeneration the tails were 

 clipped as soon as part of the previously removed tips had regenerated 

 (at 47, 61, 68, 82, 96, 110, and 124 days after hatching). 



The result is summarized in Table II. It was practically the same as 

 that of the first experiment. The larvas of Series LVI metamorphosed 

 (i.e. shed the skin for the first time) 6 days later than the controls. 

 Since this difference is smaller than the differences observed among 

 the larvae of the same series it may be said that in both series the 

 larvae metamorphosed at the same time. And certainly there was 

 no neoteny produced by removal of even considerable amounts of 

 tissue (42.4 mm. of tail were removed by the successive clippings of 

 the whole larval period), though the species used is one which would 

 be expected to yield readily to influences producing neoteny in a 

 species like Salamandra maculosa, which only rarely is found in 

 neotenous condition, 



CONCLUSIONS. 



It is apparently quite certain that removal of parts of the body 

 (limbs, tail) followed by regeneration of these parts (1) does not 

 produce neoteny in the larvae of salamanders, and (2) has no influence 

 upon metamorphosis. 



