JOHN H. NORTHROP 



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TABLE VI. 

 Effect of Adding Increasing Amounts of Peptone to Pepsin Solution. 

 0.5 cc. of 5 per cent active pepsin solution + noted cc. of peptone solution 

 (from digested egg albumin) and made up to 10 cc. K = 8.5 (Table III), E = 

 3.16, d = 0.85 per cc. of peptone solution. 



peptone added should have a greater effect than the second, the sec- 

 ond a greater than the third, and so on; the relative decrease of the 

 effect depending on the value of the equilibrium constant. Table 

 VI and Fig. 4 give the result of an experiment carried out in this way. 

 It will be seen that the effect of adding increasing units of peptone 

 agrees very well with the calculated values. The compound pepsin- 

 peptone is widely dissociated at this dilution inasmuch as with a 

 total concentration of 0.85 units of peptone and 3.16 units of pepsin 

 only 0.28 units are combined. This fact is shown graphically in 



