H. C. HAMPTON AND L. G. M. BAAS-BECKING 



641 



Peroxide quantities are proportional to reaction time, as the last 

 two columns show. No linear relation exists, however, between the 

 former and the reaction velocity. Fig. 3 shows that this relation can 

 be expressed by a continuous curve which is not exponential, as the 

 following calculation indicates. 



There seems to be no simple connection between peroxide quantity 

 and reaction velocity. The laws derived by Schutz-Borissow, 

 Issajew, and Herzog do not hold here. 



This is otherwise with enzyme quantity. Three curves, run with 

 3 cc. of neutralized peroxide and different amounts of enzyme gave 



* K calculated after 414 sec. 



According to the low strength of the enzyme, the peroxide is very 

 much in excess, and therefore the reaction velocity is proportional 

 to the enzyme concentrations, not to the peroxide concentrations. 

 Senter found exactly the same to be the case with blood catalase 

 (see also the more recent paper by Yamasaki). The values obtained 

 with hemp gave a proportionality to both peroxide and enzyme 

 quantity. This is probably due to the great strength of the hemp 

 catalase. However, it is remarkable that the reaction velocity still 

 remains in a Hnear relation to the peroxide quantities. We have no 



