30 



JOURNAL OF THE 



[April, 



V. Supply Can for Sterilized Non-aerated Water. 



A little flask, fitted as a wash-bottle, will usually suffice to 

 supply a small quantity of water, recently boiled, cooled, and free 

 from dissolved air. 



But when there is need of a less fragile apparatus for more con- 

 stant or larger supply, and, especially in bacteriological research, 

 it is desirable to have at hand a reservoir of sterilized water, the 

 following apparatus will be of service. A cylindrical can (Fig. 3), 

 made of copper or tin, and of any desired capacity, is covered 

 with a tightly fitting cap, which can be removed for cleaning the 



Fig. 3. 



interior. In the centre of the cap an automatic escape-valve (V) 

 for steam is inserted. From the side of the can, at the bottom, 

 a supply-pipe runs out a few inches horizontally, ending in a fau- 

 cet. The can, thoroughly cleaned and scalded out, is nearly filled 

 with distilled water and heated over a burner to boiling, for a 

 couple of hours. While the steam is actively escaping from the 

 valve, a wad of sterilized cotton (W) is quickly wrapped about 

 the valve, and the burner removed. The wad is fastened with a 

 turn of a piece of wire, and a cone of filter paper thrust over the 

 end of the supply-pipe, to protect it from dust. When sterilized 

 water is needed, the paper cone is removed, the end of the pipe 

 flamed, and the faucet turned. 



VI. Mountino^ Medium for A/gce and Fungi. 

 The microscopic objects and structures which receive the at- 



