96 



ION SERIES AND PROTEINS. 



ence as the corresponding curves for gelatin in Fig. 1. Fig. 6 gives 

 the curves for the influence of the two acids upon the osmotic pressure 

 of 1 per cent solutions of originally isoelectric albumin. The two 

 curves are identical and are also identical with those of albumin chlor- 

 ide and albumin phosphate in Fig. 4, thus confirming our theory. 



In Fig. 7 are given the curves for combining ratios of NaOH, 

 Ca(0H)2, and NH4OH with isoelectric albumin. The curve for 

 Ca(0H)2 is identical with that for the strong base NaOH, indicating 

 that Ca(0H)2 combines with egg albumin in equivalent proportions. 



200 

 180 



d 140 

 § 120 

 ^ 100 



Q 



I 



80 



60 



40 



20 





 pH 1 2 3 4 5 6 



Fig. 6. Curves of osmotic pressure of solutions of albumin acetate and albumin 

 dichloracetate. The curves for both acids are identical. 



Fig. 8 shows that the curve for the osmotic pressure of Ca albuminate 

 is only one-half as high as that of Na albuminate as was to be ex- 

 pected. The curve for NH4 albuminate is identical with that for 

 Na albuminate, which was to be expected since the NH4 is monovalent. 

 The results with albumin are therefore identical with those obtained 

 in the case of the corresponding gelatin salts. The result that gelatin 

 phosphate and albumin phosphate behave Hke gelatin chloride and 

 albumin chloride may be considered as a crucial test against the col- 

 loidal conception of ion effects on proteins and in favor of a purely 

 chemical theory. 



