CALVIN B. COULTER 



319 



given in the form of curves, Fig. 5 being that for normal cells and Fig. 

 6 that for sensitized cells. The curves give as ordinates the gram- 

 ions X 10-^ of CI lost by the supernatant fluid, or gained by the cells. 

 It will be seen that the chlorine ion combines with both normal and 

 sensitized cells in much larger amount on the acid side of pH 4.7 than 





o 



X 



& 



6.0 5.0 4.0 3.0 pH 



Fig. 5. Curve showing the combination of normal red blood cells with chlorine, 

 ion. 5 cc. of 10 per cent cells. 



% 



o 



6.0 



5.0 



4.0 pH 



Fig. 6. Curve showing the combination of sensitized cells with chlorine ion. 

 5 cc. of 13 per cent ceUs. 



on the alkaline side. On adding NaOH, CI is actually given up by 

 the cells between pH 5.7 and 6.2. This effect cannot be due merely 

 to an increase in permeability of the cells, permitting the escape of 

 chlorine ion, for in that case the amount of chlorine ion actually found 

 should have been greatest at pH 3.5, where a trace of hemolysis 

 appeared. 



