JACQUES LOEB 



549 



the same pH and the same concentration of originally isoelectric gel- 

 atin; and the same is true for crystalline egg albumin. The reason 

 is that in the case of gelatin or albumin phosphate the anion is the 



40 



u 



14 

 12 

 10 



^ 8 





pH 14 L6 L8 2.0 2.2 24 2.6 2.8 3.0 3.2 34 36 3.8 



Fig. 1. Ordinates represent the cc. of 0.1 N HCl or H3PO4 in 100 cc. of 1 per 

 cent casein solution. The abscissae are the pH of the solution. Approximately 

 three times as many cc. of 0.1 n H3PO4 as of 0.1 N HCl are required to bring 1 

 gm. of casein to the same pH. 



