552 



CHEMICAL AND PHYSICAL BEHAVIOR OF CASEIN 



We had shown that when we add Ca(0H)2 or Ba(0H)2 to isoelectric 

 gelatin or isoelectric crystalline egg albumin the two alkalies combine 

 with the protein in equivalent proportion.^ Hence the same number 



40 



38 



36 



C 34 



32 

 30 

 28 

 26 

 24 

 22 

 20 

 18 



16 



•S 14 



•r-i 



^ 12 





o 



o 

 in 



o 

 u 



o 



<0 



I— I 

 o 



u 

 o 



10 

 8 

 6 

 4 



pH 6 



8 



10 



11 



12 



Fig. 4. Ordinates are the cc. of 0.1 n NaOH, KOH, Ca(0H)2, and Ba(0H)2 in 

 100 cc. of 1 per cent solution of casein. Abscissae are the pH of the solution. 

 The curves for the four alkalies are identical, proving that Ba and Ca combine 

 with casein in equivalent proportion. 



