654 



LATENCY TIME IN ENZYME DETERMINATION 



the reaction was completed in 2,058 seconds (Fig. 1). The abscissa 

 represents the time; the ordinate the amount of substance decomposed 

 under the influence of the enzyme. Let A' be the time when the 

 enzyme is added, A the time when the reaction starts, and A' -A 

 the latency time T°,A' - P = T'; A' - Q = T". If the reaction is 

 monomolecular, the equation of the line yl.SC will be 



1 a 



K = -In 



T a — X 



Fig. 1. 



in which K represents the reaction velocity, T the time, a the avail- 

 able amount of substrate, and x the amount decomposed. If the 

 reaction is supposed to be polymolecular the following formula will 

 apply. 



K = 



aP- — {a — x)'^ 

 T.a" (a — x)" 



in which w-1 is the order of the reaction. 

 The proportion of T' and T" will be 



T' a -x' 



In 



(1) 



