HENRY SCHMITZ 



797 



Carhohydrases. 



The action of carbohydrases was determined on 1 per cent solu- 

 tions of maltose, lactose, sucrose, raflinose, potato starch, inulin, 

 white fir cellulose, and hemicellulose from date seed endosperms. 

 After varying periods of incubation, the cultures were filtered and 

 5 cc. samples of the filtrates treated with 20 cc. of Fehling's solution. 

 The results in the following table are the average of two titrations 

 and are expressed as the number of cubic centimeters of 0.05 n 

 potassium permanganate solution required to oxidize the dissolved 

 copper, 



TABLE I. 



Carbohydrase Action of Polyporus volvatus and Fomes igniarius. 



Incubation 

 period. 



days 



14 



20 



3 



4 



4 



10 



30 



30 



Substrate. 



Maltose 



Lactose 



Sucrose 



Raffinose 



Potato starch. . . . 



Inulin 



White fir cellulose 

 Hemicellulose. . . . 



Polyporus volvatus. 



With 



fungous 



meal. 



With 



fungous 



meal auto- 



claved. 



Without 

 fungous 

 meal. 



Fomes igniarius. 



With With 



•"^^^- claved. 



0.05 N KMnOi 



17.15 

 22.00 

 6.00 

 4.35 

 3.55 

 5.00 

 4.95 

 5.25 



Glucosidase. 



The presence of a glucosidase in the fungous meal of the two 

 fungi here discussed was determined by the action of the fungous 

 meal upon a 1 per cent solution of salicin. After a 5 day incuba- 

 tion period, the enzyme cultures were filtered and sugar determina- 

 tions made of 5 cubic centimeter samples of the filtrates. The results, 

 expressed in the number of cubic centimeters of 0.05 N potassium 

 permanganate solution required to oxidize the dissolved copper are 

 shown in Table II. 



