196 Journal of Mycology [Vol. 13 



from Wvmore, Neb., was sown on Onagra biennis May 19, giving 

 rise to pycnia May 26, and aecia June 2, both in abundance.^^ 



6. PucciNiA ALBiPERiDiA Aith. — This rust on three species 

 of hosts was obtained in different locaHties near Lafayette. Ind., 

 and sown with the following results : 



From Carex squarrosa L., sown in greenhouse April 16 on Ribes rotundi- 



folium; April 21, pycnia; April 30, aecia. 

 From C. squarrosa L., sown in garden April 21 on R. gracile; April 25, 



pycnia ; May 13, aecia. 

 From C. squarrosa L., sown in greenhouse April 16 on R. rubrum ; no 



infection. 

 From C. tetanica Schk., sown in greenhouse April 20 on R. Cynosbati; 



April 27, pycnia; May 9. aecia. 

 From C. critiita Lam., sown on R. Cynosbati in greenhouse April 26, then 



plant transferred to garden ; May 4, pycnia ; May 17, aecia. 



These results add one more telial host to those previously 

 used for cultures.^* They also have given an opportunity for 

 a study of the differences between the pale aecia obtained by 

 cultures and the highly colored aecia usually observed in the field. 

 The aecia grown wholly in the greenhouse were pale, as in pre- 

 vious years ; those on the plant which had the pot plunged into 

 the garden soil after the fungus became established, were much 

 more colored ; and those raised from sowings made in the gar- 

 den were highly colored and presented essentially the same 

 appearance as others that came upon some nearby bushes of 

 Ribes from natural infection. The result of observations during 

 the last six years, coupled with the cultures of this year, make 

 the conclusion almost inevitable that shade, moist air, and slow 

 growth, tend to make the aecia smaller, wdth less coloring mat- 

 ter in the peridial cells and surrounding mycelium, and also 

 tend to produce less hypertrophy of the tissues of the host, 

 and that this accounts for the differences observed between aecia 

 grown in cultures and those very common on Ribes Cynosbati, 

 R. rotnndifoliuin, R. gracile, and similar species of gooseberries 

 throughout tlie eastern United States. All collections of this 

 sort, therefore, may be called Pnccinia albipcridia, but whether 

 this is a distinct species from the very similar rust of Europe, 

 Puccinia Grossulariae (Schum.) Lagerh., or one of the several 

 biological .species e.stablished by Klebahn, still remains an open 

 question. 



7. Puccinia angustata Peck. — Teliosporic material on 

 Scirpus atrovirens Muhl., from the vicinity of Lafayette, Ind., 

 was sown April 28, on Dirca palustris, with no infection. On 



"For previous cultures see Bot. Gaz. J5:13. 1903; Jour. Myc. 5:55. 

 1902; //:5H. 1!)0.5; and I2:\r>. IWH. 



"For previous cultures see Jour. Myc. 5:53. 1902; /o:ll. 1904; 

 //:58. 1905; and /^:14. 1906. 



