10 Journal of Mycology [Vsl. 14 



Still a third collection made at Boulder, Colo., by the writer, was 

 sown on Hydro phylhtm cap'xtatum, Lactiica Canadensis, Iris ver- 

 sicolor, Phrynia Icptostachya, Artemisia serrata, and A. dracun- 

 culoides. None of these sowings gave infection. Similar mate- 

 rial had been tried on five of these hosts before, and on twenty- 

 nine other species, all with negative results.* The rust on this 

 host is common in the northern states east of the Rocky moun- 

 tains, and appears from its morphological characters to be distinct 

 from any described form. Fifty-five sowings have now been 

 made, beginning in 1903, using forty-three species of hosts, and 

 as no innoculation has been effected, the necessity for careful 

 field observations to detect the probable alternate host becomes 

 highly imperative in order to determine the real life cycle. 



2. PucciNiA on Carex gravida Bailey, sent twice by Rev. 

 J. M. Bates, first from St. Paul, Neb., was sown on Aesculus 

 glabra, Viola ciicullata, Ribcs Cynosbati, and Hydrophyllum 

 Virginicum, and second from Red Cloud, Neb., was sown on 

 Hypoxis erecta, Housfonia purpurea, Laciniaria spicata, Lactuca 

 Canadensis, and Artemisia dracunculoides, with no infection. 

 Similar material from the same region has been sown in previous 

 years upon thirty-four other species of hosts with negative 

 results.^ 



3. Plccinia on Carex Douglasii Boott. collected at Valen- 

 tine, Neb., by Rev. J. M. Bates, was sown on Symphoricarpus 

 racemosus, Kapaea dioica, Psoralea Onobrychis, Aesculus glabra, 

 Laciniaria spicata, Hypoxis erecta, and Flacata comosa, with no 

 infection. 



4. PuccixiA Ellisiana Thuem., on Andropogon scoparius 

 Michx., collected at Boulder, Colo., by Mr. E. Bethel, was sown 

 on Arabis sp., Thalictrum dioicum, Actaea alba, Psoralea Onobry- 

 chis, Poly gala Senega, J'iola striata, Polemonmm reptans, Sol- 

 idago Canadensis, Boltonia asteroides, Senecio obovatus, Le- 

 pachys pinnata, and Smilocina stellata, with no infection. 



5. Puccini A viijoata Ellis & Ev., on Chrysopogon airnacens 

 (Alichx.) Benth., collected at Grand Island, Neb., by Rev. J. 

 M. Bates, was sown on Ceanotlms Americana, Napaea dioica, 

 Caidophyllum thalictroides, and Cassia Chamaecrista. with no 

 infection. 



'See Jour. Myc. /o:10. 1904; /7:51. 1905; I2:\2. 1906; and 

 /i:191. 1907. 



"See Jour. Myc. 7o:10. 1904 77:52. 1905; and 7j:191. 1907. 



