Jan. 1908] Cidtures of Uredineae in igoy 15 



6. PucciNiA suBNiTENS Diet.— Teliosporic material on 

 Dishchlis spicata (L.) Greene was sent by Rev. J. M. Bates, from 

 Red Cloud, Neb., and sown as follows: 



April 10 on Chenopodium album; April 19, pycnia; April 23 aecia 

 April 10 on Bursa Bursa-pastoris; April 26, pycnia; May 1 'aecia.' 

 April 10 on Sarcobatus vcrmiculatus ; no infection. 

 April 17 on Chenopodium album; April 28, pycnia; May 1, aecia 

 April 17 on Sarcobatus vcrmiculatus (two plants) ; no infection. 

 April 25 on Chenopodium album; May 6, pycenia; May 8, aecia'. 

 April 25 on Sarcobatus vcrmiculatus; no infection. 



Another collection of the rust on the same host, obtained by 

 the writer at Ogallala, Neb., was sown on Chenopodium album 

 April 29, giving rise to pycnia May 9, and aecia May 13. A 

 sowing at the same time on Sarcobatus vermiculatus, and again 

 on two plants June i, gave no inefction. It may be definftely 

 concluded that this species of rust as it exists in Nebraska is not 

 transferable to Sarcobatus vermiculatus}^ 



7. PucciNiA AMPHiGENA Diet.— Tcliosporic material on 

 Calamovilfa longifolia (Hook.) Hack., collected at Burnett, Neb., 

 by Rev. J. M. Bates, was sown on Smilax hispida May 2, giving 

 rise to pycnia May 9, and aecia May 16." 



8. PucciNiA Phragmitis (Schum.) Korn.— Teliosporic 

 material on Phragmites communis Trin., collected at Scotia Junc- 

 tion, Neb., by Rev. J. M. Bates, was sown on Rum ex crispus 

 May I, givmg rise to pycnia (date not noted) and aecia May ly}^ 



9. PucciNiA siMiLLiMA ^r//j.— Teliosporic material on 

 Phragmitis communis Trin., collected at Scotia Junction, Neb., 

 by Rev. J. M_. Bates, was sown on Ruinex crispus, with no infec- 

 tion, being mistaken for the previously mentioned species. Later 

 it was sown on two plants of Anemone Virginiana, still with no 

 infection. It was then sown on Anemone Canadensis June 3, 

 giving rise to abundant pycnia June 11, and aecia June 19. This 

 is a confirmation of the restricted range of aecial host, only one 

 species of Anemone yet being found that can be infected. ^« 



10. PucciNiA Agropyri Ellis & Ev.— This is a very com- 

 mon rust of the Rocky mountain region. No culture of it has 

 ever been tried in this country, but a collection made at Bozen, 

 in the Austrian Tyrol, was successfully sown on Clematis Vitalba, 



"For previous cultures see Bot. Gaz. J5:19. 1903; Jour. Myc. 77:54. 

 1905; 7^:16. 1906; and 7i:197. 1907. 



"For previous cultures see Bot. Gaz. j'?:20. 1903 ; Tour. Myc. 77:57 

 1905; and 7^:16. 1906. . ^ j 



'"For previous cultures see Bot. Gaz. ^9:269. 1900; and Jour. Myc 

 9:220. 1903. 



" For previous cultures see Bot. Gaz. 35:20. 1903. 



