20 fournal of Mycology [Vol. 14 



Roland Thaxter,^- in an extensive series running from i! 

 to 1890, inclusive. He grew it on Amelanchier Canadensis in 

 great abundance, showing both pycnia and aecia, and in one 

 instance pycnia were formed on the common apple, but failed 

 to develop further. Thaxter also noted the early development 

 of pycnia and aecia. 



The following eight species have now been grown in cultures 

 for the first time, so far as the writer knows. The two cases 

 of amphispores and the one autoecious species resulted as any- 

 one might have taken for granted, but in the other five cases 

 the results are wholly unpredicted, and represent very material 

 advancement in the knowledge of American heteroecious rusts. 



1. Puccini A vexans Farl. — Material bearing both telio- 

 spores and amphispores on Atheropogon curtipendiilus (Michx.) 

 Fourn. {Bouteloua raceniosa Lag.), was sent from Boulder, 

 Colo., by Air. E. Bethel, and gave good germination for the 

 amphispores, but the teliospores refused to grow. Sowing was 

 made on Atheropogon ciirtipendulns May 29, and uredinia were 

 observed June 21, although they may have appeared earlier and 

 been overlooked. The amphispores were the characteristic, dark- 

 colored, thick-walled and four-pored form, while the uredinio- 

 spores, to which they gave rise, had the usual light-colored, thin- 

 walled anr eight-pored appearance. The difference between the 

 resting form (amphispore) and active form of the urediniospores 

 belonging to this species is very striking. This is the second 

 time, as far as the writer knows, that amphispores of this species 

 have been seen to germinate. 



2. PucciNiA Cryptandki ElHs & Barth. — Amphisporic 

 material on Sporohohis cryptandnts (Torr.) A. Gray, obtained 

 at Manitou, Colo., by Mr. F. D. Kern and the writer, was sown 

 on a plant of the same species of grass May 9, and uredinia 

 appeared May 24. In this case the difference between the resting 

 or amphisporic form of the urediniospores and the active or 

 summer form is not so marked as in the precetling species, and 

 yet there is no mistaking one for the other. 



3. PucciNiA OBTECTA Peck. — Teliosporic material on 

 Scirpus Americanus Pers., obtained near Lafayette, Ind., by Mr. 

 F. D. Kern, was sown on Urtica gracilis, and Silphiitm perfoli- 

 atuni, with no infection, and afterward on Bidens frondosa June 

 5, givin--- rise to a few pycnia June 19, and aecia first noticed 

 July 6. but probably opening earlier. Another sowing was made 

 on B. frondosa and also on B. connata June 24, both showing a 

 good devedopment of pycnia July i, and aecia July 6. 



''Bot. Gaz. 77:238. 1886; Proc. Am. Acad. Sci. 22:2QA. 1887; Bot. 

 Gaz. 7^:167. 1889; and Bull. Conn. Exper. Sta. 707:6. 1891. 



