19121 A Study of the Action of Diuretics 7 



Caffeine 1-2 cc. of a 1 per cent solution per kilogram 



Theobromine .... 1-2 cc. of a 1 per cent solution per kilogram 



Digitalin 1 mg. per kilogram 



Sodium chloride solution. . . .0.9 per cent, 10 cc. per kilogram 



COURSE OF THE EXPERIMENTS 



The average daily output of urine of each animal was deter- 

 mined at the end of the third day, during which time the pre- 

 liminary observations were being made. Following the injec- 

 tion of uranium the daily output of urine was ascertained and 

 compared with the average daily output by the animal prior 

 to the use of uranium. 



Three of the animals were used experimentally after they had 

 developed a nephritis but before the development of a gly- 

 cosuria. The daily output of urine by these nephritic and non- 

 glycosuric animals showed a moderate increase as follows. The 

 urine from the different animals increased respectively from 

 278 to 318 cc, from 392 to 440 cc. and from 386 to 358 cc. 

 The urine showed qualitatively a pronounced reaction for albu- 

 men, and microscopically hyaline and granular casts and ery- 

 throcytes. 



The remaining animals were used experimentally after the 

 development of a glycosuria. In each instance, with the devel- 

 opment of a glycosuria the output of urine at once enormously 

 increased. For example, in experiment 1, in which the animal 

 was receiving daily 350 cc. of water, the average daily output 

 of urine for three days prior to the uranium was 385 cc, while 

 with the development of a nephritis and an accompanying gly- 

 cosuria the urine increased on the first day to 620 cc. and on 

 the second day to 750 cc 



Again, in experiment 8, in which the animal was receiving 

 500 cc. of water daily, the average output of urine prior to the 

 uranium was 513 cc, while following the uranium with the 

 development of a nephritis and a glycosuria the output of 

 urine increased to 1310 cc. 



This increase in the output of urine was not an occasional 

 occurrence, but it developed in each animal that was allowed a 

 sufficient time to develop a glycosuria. These polyuric and 

 nephritic animals were anesthetized by one of the methods pre- 



